LOCUS       sequence-313366-        2493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses TMEM63A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2493)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMEM63A_OE_1
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2423..2447)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2449..2490
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgatggact ccccgttcct ggagctgtgg cagtccaagg cagtgtccat cagggagcag
       61 ctgggactcg gggaccggcc caacgactcc tattgctaca actcggccaa aaacagcacc
      121 gtgctccagg gggtcacctt tggtggcatc cccactgtcc tgctcataga cgtcagctgc
      181 ttcctgttct taatcttggt gttttctatt ataagaagaa gattctggga ctatggccgc
      241 attgccctgg tgtcagaagc agacagcgag tccagatttc agagattgtc atcgacttcc
      301 tcctcaggtc aacaagactt tgaaaatgag ctgggatgct gtccctggct gactgccatc
      361 ttccgtctgc atgatgacca gatcctggaa tggtgtgggg aggacgccat ccactacctg
      421 tccttccaga ggcacatcat cttcctgttg gtggtggtca gctttttgtc cctgtgtgtc
      481 atcctgcctg tcaacctctc aggggacttg ctggacaaag acccgtatag ttttgggagg
      541 acaacaatag caaacctaca gactgacaat gacctccttt ggctgcacac catctttgct
      601 gtcatttacc tcttcctcac tgtgggtttc atgcggcacc acactcagtc cattaagtac
      661 aaagaggaga acctggtgag gcggaccctg ttcatcacag gactccccag agatgccagg
      721 aaggagactg tggagagcca cttccgggac gcgtatccca cgtgtgaggt ggttgatgtg
      781 cagctgtgct acaacgtggc caaactgatc tacctgtgca aggagaaaaa gaagactgag
      841 aagagcctga cctattacac aaacctgcag gtgaagacag gccagcggac cctcatcaac
      901 cccaagccct gtggccagtt ttgctgctgt gaagtgctgg gctgtgagtg ggaagacgcc
      961 atctcttact acacacggat gaaggacagg ctgctggaga ggatcacaga ggaagaacgc
     1021 cacgtccagg accagcccct gggaatggcc ttcgtcacct tccaggagaa gtccatggcc
     1081 acctacatcc tgaaagattt caatgcctgc aagtgtcaga gccttcagtg caaaggtgag
     1141 ccccagccgt cctcccatag cagggagctc tatacctcca agtggacagt cacctttgct
     1201 gctgaccctg aggacatctg ctggaagaac ctctctatcc agggcctccg ctggtggcta
     1261 cagtggctgg gcatcaactt caccctcttc ctggggctat ttttcctgac cacaccctcc
     1321 atcatcctgt ccaccatgga caagtttaat gtcaccaaac ccatccatgc gctgaataac
     1381 ccgatcatca gccagttctt ccccaccctc ctgctctggt ccttctcggc cctgctcccc
     1441 tccattgtct actactctac actgctggag tctcactgga ccaagtcggg ggaaaaccag
     1501 atcatgatga ccaaagtcta catattcttg atcttcatgg tgctgatcct gccctccctg
     1561 ggtctcacca gtctagattt tttcttccgg tggctctttg acaaaacttc ctcggaggcc
     1621 tccatcaggt tggagtgcgt cttcctgcct gaccagggtg ccttctttgt gaactatgtc
     1681 atcgcctcgg ccttcatcgg caatggcatg gagctgctgc ggctgccagg tctcatcctc
     1741 tataccttcc gcatgatcat ggccaagacg gctgctgacc gcaggaatgt caagcagaac
     1801 caggccttcc agtacgagtt tggagccatg tatgcatgga tgctgtgtgt cttcactgtc
     1861 atcgtggcct acagcatcac ttgtcccatc atcgcgccat ttggcctcat ctacatcctg
     1921 ctcaagcaca tggtggaccg gcacaacctc tacttcgtct acctcccagc caagctggag
     1981 aaggggatcc actttgccgc tgtgaaccag gccttggcag cccccatcct gtgcctcttc
     2041 tggctctact tcttttcctt cctgcgcctg ggtatgaagg cccccgccac tctgttcacc
     2101 ttcctggtgc tgctgctcac catcctggtc tgcctggctc acacctgctt tggatgcttc
     2161 aagcacctca gccctctgaa ctacaaaaca gaagagcctg caagtgacaa aggaagtgag
     2221 gcagaggccc acatgccccc accgttcaca ccctacgtgc ctcggattct gaacggcttg
     2281 gcctcggaga ggacagcact gtctccgcag cagcagcagc agcagaccta tggtgccatc
     2341 cacaacatca gcgggactat ccctggacag tgcttggcgc agagcgccac gggcagtgtg
     2401 gctgctgccc cccaggaggc ctgcccaact ttcttgtaca aagtggttgg taagcctatc
     2461 cctaaccctc tcctcggtct cgattctacg tag
//
