LOCUS       sequence-313376-        1461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses GLRA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1461)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GLRA3_OE_1
FEATURES             Location/Qualifiers
     source          1..1461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1391..1415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1417..1458
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggcccacg tgagacactt tcggacatta gtttcgggat tttacttctg ggaagcagca
       61 ctgttactca gtttggttgc cacaaaggaa acagacagtg caagatctcg aagtgctcca
      121 atgtcacctt ctgattttct ggataaatta atgggcagga catcaggata tgatgcaaga
      181 atcagaccca attttaaagg ccctccagtt aatgtcacat gcaacatatt catcaacagc
      241 tttggctcta tcgcagagac gaccatggat tacagagtga atatctttct tcgtcagaaa
      301 tggaatgatc cccgcctcgc gtacagtgaa tatcctgacg actctttaga cctcgacccc
      361 tccatgttgg actccatttg gaaacctgat ttgttctttg ccaatgaaaa gggtgccaac
      421 tttcatgaag tcactacaga caacaaattg ctaagaattt tcaaaaatgg aaatgttctt
      481 tattcaataa gattaacatt aacactttcc tgtccaatgg atctcaagaa ttttcccatg
      541 gatgtacaaa catgtataat gcaactggaa agctttgggt acacaatgaa tgatctcatt
      601 tttgaatggc aagatgaggc acccgtacaa gtggcagaag gactcacttt gccccagttt
      661 ctgttgaaag aagaaaaaga tttacgatac tgcactaaac attacaatac aggaaagttt
      721 acgtgtatag aagtgcgatt ccatctggag cgacaaatgg gatactatct gatccagatg
      781 tacattccca gtctcctgat tgttattcta tcctgggttt cgttctggat caacatggat
      841 gcagcaccgg ccagggtagc tctggggata accaccgtgc taacgatgac tacacagagt
      901 tcaggatcac gagcttcctt gccaaaagtt tcatatgtca aagctattga tatttggatg
      961 gcagtatgcc tcctttttgt gttttcagca cttctggagt atgcagctgt aaattttgta
     1021 tcaagacaac acaaagaact tctgagattt cgacgaaaga gaaagaataa gacagaagcc
     1081 tttgcactgg agaagtttta ccgtttctca gatatggatg atgaggtaag ggaaagccga
     1141 ttcagcttca cagcctatgg aatgggacca tgtctacaag caaaggatgg catgactcca
     1201 aagggcccca accaccctgt ccaggtaatg ccaaaaagtc ctgatgaaat gaggaaggtc
     1261 tttatcgacc gggccaagaa gattgatacc atctcccgag cctgcttccc attagctttt
     1321 ttgattttta atattttcta ctgggttatc tataaaattc ttaggcatga ggatattcat
     1381 cagcagcaag atacagcttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     1441 ctcggtctcg attctacgta g
//
