LOCUS       sequence-313377-        1488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses BEST4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_BEST4_OE_1
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1418..1442)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1444..1485
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgacggttt catacactct caaagtggcg gaggcccgct tcggaggttt ctctggcctg
       61 cttctccgct ggaggggaag catctacaag ctcctctaca aggaattcct cctctttggg
      121 gccttgtacg ctgtgcttag catcacctac cggctgctgc tgacccagga gcagaggtac
      181 gtgtatgctc aggtggcccg gtactgcaac cgctcagcag acctcattcc cttgtccttt
      241 gtattgggtt tctatgtgac tctcgtggtg aaccgctggt ggtcccagta cacaagcatc
      301 ccgctgccag accagctgat gtgcgtcatc tcggctagcg tgcacggcgt ggaccagcgg
      361 ggccgcctgc tgcgccgcac cctcatccgc tacgcgaacc tggcgtccgt gctggtgctg
      421 cgctcggtca gcacccgcgt gcttaagcgc ttccccacca tggagcacgt ggtggatgca
      481 ggtttcatgt cccaggaaga gaggaaaaag tttgagagcc tgaaatccga cttcaacaag
      541 tactgggtcc cctgcgtctg gttcaccaac ctggcggccc aggcccggag ggacgggcga
      601 atacgtgacg atatcgctct ctgtctactt ttggaagagc tgaacaagta ccgagccaag
      661 tgcagcatgc tattccacta tgactggatc agcatccccc tcgtctacac ccaagtggtg
      721 accatagccg tctactcttt ctttgccctc tccctggttg gccgccagtt tgtggagcca
      781 gaggcagggg ctgccaaacc tcagaagctt ctgaagccag gccaggagcc agccccagcc
      841 ctgggagacc cggacatgta cgtgcctctc accactctgc tgcagttctt cttctatgct
      901 ggctggctca aggtggctga acagatcatc aacccatttg gtgaggatga tgacgacttt
      961 gagacaaatc agctcataga ccgcaacttg caggtgtccc tgctatccgt ggacgaaatg
     1021 taccagaacc ttccccccgc tgagaaggac cagtactggg atgaggacca gccgcagcca
     1081 ccctacactg tggccacggc ggccgagtct ctgcggccct cattcctggg ctccaccttc
     1141 aacctgcgca tgagcgacga ccctgagcag agcctgcagg tggaggcgtc ccccggatct
     1201 ggtcggcccg cgcccgccgc gcagaccccg ttgctcggcc gcttcctggg cgtaggggcg
     1261 ccctccccgg ccatcagcct ccggaacttc ggccgcgtgc gaggcacccc ccgccccccg
     1321 catctgctgc gcttccgggc ggaggagggc ggcgaccccg aggccgcagc ccgcatcgag
     1381 gaggaatcgg cggagtccgg ggacgaggcc ctggagccca cagctttctt gtacaaagtg
     1441 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtag
//
