LOCUS       sequence-313379-        1437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNA7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1437)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNA7_OE_1
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1367..1391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1393..1434
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggagccgc ggtgcccgcc gccgtgcggc tgctgcgagc ggctggtgct caacgtggcc
       61 gggctgcgct tcgagacgcg ggcgcgcacg ctgggccgct tcccggacac tctgctaggg
      121 gacccagcgc gccgcggccg cttctacgac gacgcgcgcc gcgagtattt cttcgaccgg
      181 caccggccca gcttcgacgc cgtgctctac tactaccagt ccggtgggcg gctgcggcgg
      241 ccggcgcacg tgccgctcga cgtcttcctg gaagaggtgg ccttctacgg gctgggcgcg
      301 gcggccctgg cacgcctgcg cgaggacgag ggctgcccgg tgccgcccga gcgccccctg
      361 ccccgccgcg ccttcgcccg ccagctgtgg ctgcttttcg agtttcccga gagctctcag
      421 gccgcgcgcg tgctcgccgt agtctccgtg ctggtcatcc tcgtctccat cgtcgtcttc
      481 tgcctcgaga cgctgcctga cttccgcgac gaccgcgacg gcacggggct tgctgctgca
      541 gccgcagccg gcccgttccc cgctcggctg aatggctcca gccaaatgcc tggaaatcca
      601 ccccgcctgc ccttcaatga cccgttcttc gtggtggaga cgctgtgtat ttgttggttc
      661 tcctttgagc tgctggtacg cctcctggtc tgtccaagca aggctatctt cttcaagaac
      721 gtgatgaacc tcatcgattt tgtggctatc cttccctact ttgtggcact gggcaccgag
      781 ctggcccggc agcgaggggt gggccagcag gccatgtcac tggccatcct gagagtcatc
      841 cgattggtgc gtgtcttccg catcttcaag ctgtcccggc actcaaaggg cctgcaaatc
      901 ttgggccaga cgcttcgggc ctccatgcgt gagctgggcc tcctcatctt tttcctcttc
      961 atcggtgtgg tcctcttttc cagcgccgtc tactttgccg aagttgaccg ggtggactcc
     1021 catttcacta gcatccctga gtccttctgg tgggcggtag tcaccatgac tacagttggc
     1081 tatggagaca tggcacccgt cactgtgggt ggcaagatag tgggctctct gtgtgccatt
     1141 gcgggcgtgc tgactatttc cctgccagtg cccgtcattg tctccaattt cagctacttt
     1201 tatcaccggg agacagaggg cgaagaggct gggatgttca gccatgtgga cacgcagcct
     1261 tgtggcccac tggagggcaa ggccaatggg gggctggtgg acggggaggt acctgagcta
     1321 ccacctccac tctgggcacc cccagggaaa cacctggtca ccgaagtgac agctttcttg
     1381 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtag
//
