LOCUS       sequence-31338-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C6orf197
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttccctctga atattttgtt taactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcatg accgttcgtg agaaaacgcg
      121 cctggaaaaa tttcgtcagc tgctgtcgtc gcaaaacacc gacctggatg aactgcgcaa
      181 atgtagttgg ccaggcgtcc cgcgtgaggt gcgccctatc acttggcgtc tgctgtcagg
      241 ttatctgccg gcgaatacag aacgccgtaa actgacgctg cagcgcaaac gtgaggaata
      301 cttcgggttt attgaacaat attacgacag tcgtaatgag gaacatcacc aggataccta
      361 tcgtcagatc catattgata tcccacgcac taatccgctg attcctctgt tccaacagcc
      421 gctggtacaa gagatctttg aacgtattct gtttatctgg gccattcgcc acccagcctc
      481 gggatacgtt cagggcatta acgacctggt gacaccgttc tttgtcgttt tcctgtcgga
      541 atatgtagag gaagatgtgg aaaattttga cgtcaccaac ctgtctcagg atatgctgcg
      601 tagtatcgag gcggatagct tctggtgcat gtcaaaactg ctggacggta ttcaagataa
      661 ttatacgttt gctcagcctg gcatccaaaa aaaagttaaa gccctggaag agctggtgtc
      721 ccgcattgac gaacaggtac ataaccactt ccgtcgctac gaagtcgagt atctgcagtt
      781 tgcattccgt tggatgaata acctgctgat gcgcgaactg ccactgcgtt gtaccatccg
      841 cctgtgggat acttaccaat cggagccgga aggtttttct cattttcacc tgtatgtttg
      901 cgcagcttct g
//
