LOCUS       sequence-313380-        1647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses KCNS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1647)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNS1_OE_1
FEATURES             Location/Qualifiers
     source          1..1647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1577..1601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1603..1644
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgctgatgc tgctggtccg gggaacacac tatgagaacc tccggtctaa agtggtgctg
       61 ccaacacccc taggagggag gagcactgaa acctttgtga gcgagttccc gggccccgac
      121 accgggatcc gctggcggcg aagcgacgag gcgctgcgcg tgaacgtggg tggcgtgcgg
      181 cggcagctga gcgcgcgcgc cctggcgcgc ttcccgggca cgcggctggg ccgcctgcag
      241 gccgcggcgt cggaggagca ggcgcggcgc ctgtgcgacg actacgacga ggcggcgcgc
      301 gaattctact tcgaccggca cccgggcttc ttcctgagcc tgctgcactt ctaccgcact
      361 ggccacctgc acgtgctcga cgagctgtgc gtcttcgcct ttggccagga ggccgactac
      421 tggggcctag gcgagaacgc gcttgccgcg tgctgccgcg cgcgctacct ggagaggcgg
      481 ctgacccagc cgcacgcctg ggacgaggac agcgacacgc cgagcagcgt ggacccgtgc
      541 cccgacgaga tctccgacgt gcagcgagaa ctggcgcgct atggcgcggc gcgctgtggc
      601 cgcctgcgcc gccgcctctg gctgaccatg gagaacccgg gctactcgct gccgagcaag
      661 ctcttcagct gcgtctccat cagcgtggtg ctcgcctcca tcgccgccat gtgcatccac
      721 agcctgcccg agtaccaggc ccgcgaggcg gcggccgccg tggctgcggt ggccgcgggc
      781 cgcagcccgg aaggcgtgcg cgacgacccg gtgctgcgac gcctcgagta cttctgcatc
      841 gcctggttca gcttcgaggt gtcgtcgcgc ctcctgctgg cgcccagtac gcgcaacttc
      901 ttctgccacc cgctcaacct catcgacatt gtgtctgtgc tgcccttcta tctcacgctg
      961 ctggctggtg tggcactggg cgaccagggc ggcaaggagt tcggccacct gggcaaggtg
     1021 gtgcaggtgt tccgcctcat gcgcatcttc cgcgtactca agttggcgcg ccattccacc
     1081 gggctgcgct cgctgggagc cacgctcaag cacagctacc gtgaggtggg catcttgctg
     1141 ctgtacctgg ctgtgggtgt gtcagtgttc tctggtgtgg cctacacagc tgaaaaggag
     1201 gaggacgtgg gctttaacac catcccagcc tgctggtggt ggggcacagt gagcatgacc
     1261 accgtgggct atggggatgt ggtgccagtg acggtggctg gcaagctggc agcctcaggc
     1321 tgcatcctag ggggcatcct ggtggtagca ctccccatca ccatcatctt caacaagttc
     1381 tcccacttct accggcgcca gaaggctctg gaggcagccg tgcgcaacag caaccaccaa
     1441 gagtttgagg acttgctgag cagcgttgat ggggtgtcgg aggcatctct ggagacatcc
     1501 cgagaaacct ctcaggaggg acagtctgca gatctagaga gccaggcccc cagtgagcct
     1561 ccacaccctc agatgtatac agctttcttg tacaaagtgg ttggtaagcc tatccctaac
     1621 cctctcctcg gtctcgattc tacgtag
//
