LOCUS       sequence-313385-        1632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses CACNB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB4_OE_1
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1562..1586)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1588..1629
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgtcctcct cctcctacgc caagaacggg accgcggacg ggccgcactc ccccacctcg
       61 caggtggccc gaggcaccac aacccggagg agcaggttga aaagatccga tggcagcacc
      121 acttcgacca gcttcatcct cagacagggt tcagcggatt cctacacaag caggccgtct
      181 gactccgatg tctctttgga agaggaccgg gaagcaattc gacaggagag agaacagcaa
      241 gcagctatcc agcttgagag agcaaagtcc aaacctgtag catttgccgt gaagacaaat
      301 gtgagctact gcggcgccct ggacgaggat gtgcctgttc caagcacagc tatctccttt
      361 gatgctaaag actttctaca tattaaagag aaatataaca atgattggtg gataggaagg
      421 ctggtgaaag agggctgtga aattggcttc attccaagtc cactcagatt ggagaacata
      481 cggatccagc aagaacaaaa aagaggacgt tttcacggag ggaaatcaag tggaaattct
      541 tcttcaagtc ttggagaaat ggtatctggg acattccgag caactcccac atcaacagca
      601 aaacagaagc aaaaagtgac ggagcacatt cctccttacg atgttgtacc gtcaatgcgt
      661 ccggtggtgt tagtggggcc gtcactgaaa ggttacgagg taacagacat gatgcagaaa
      721 gccctctttg atttcctgaa gcacaggttt gatgggagga tttcaataac gagagtgaca
      781 gctgacattt ctcttgctaa gaggtctgtc ctaaataatc ccagcaagag agcaataatt
      841 gaacgttcga acacccggtc cagcttagcg gaagtacaaa gtgaaattga aagaatcttt
      901 gagttggcaa gatctttgca actggttgtt cttgatgcag acaccatcaa tcacccagca
      961 caacttataa agacttcctt agcaccaatt attgttcatg taaaagtctc atctccaaag
     1021 gttttacagc ggttgattaa atctagagga aagtcacaaa gtaaacactt gaatgttcaa
     1081 ctggtggcag ctgataaact tgcacaatgc cccccagaaa tgtttgatgt tatattggat
     1141 gaaaatcagc ttgaggatgc atgtgaacat ctaggggagt acctggaggc gtactggcgt
     1201 gccacccaca caaccagtag cacacccatg accccgctgc tgggaaggaa tttgggctcc
     1261 acggcactct caccatatcc cacagcaatt tctgggttac agagtcagcg aatgaggcac
     1321 agcaaccact ccacagagaa ctctccaatt gaaagacgaa gtctaatgac ctctgatgaa
     1381 aattatcaca atgaaagggc tcggaagagt aggaaccgct tgtcttccag ttctcagcat
     1441 agccgagatc attaccctct tgtggaagaa gattaccctg actcatacca ggacacttac
     1501 aaaccccata ggaaccgagg atcacctggg ggatatagcc atgactcccg acataggctt
     1561 ttgccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
     1621 gattctacgt ag
//
