LOCUS       sequence-31339-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EIF4A2
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             784..801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(854..872)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcggt gtcatcgaga gcaactggaa
      121 tgagattgtt gataactttg atgatatgaa tttaaaggag tctctccttc gtggcatcta
      181 tgcttacggt tttgagaagc cttccgctat tcagcagaga gctattattc cctgtattaa
      241 agggtatgat gtgattgctc aagctcagtc aggtactggc aagacagcca catttgctat
      301 ttccatcctg caacagttgg agattgagtt caaggagacc caagcactag tattggcccc
      361 caccagagaa ctggctcaac agatccaaaa ggtaattctg gcacttggag actatatggg
      421 agccacttgt catgcctgca ttggtggaac aaatgttcga aatgaaatgc aaaaactgca
      481 ggctgaagca ccacatattg ttgttggtac acccgggaga gtgtttgata tgttaaacag
      541 aagatacctt tctccaaaat ggatcaaaat gtttgttttg gatgaagcag atgaaatgtt
      601 gagccgtggt tttaaggatc aaatctatga gattttccaa aaactaaaca caagtattca
      661 ggttgtgttg ctttctgcca caatgccaac tgatgtgttg gaagtgacca aaaaattcat
      721 gagagatcca attcgaattc tggtgaaaaa ggaatgatga cgaagcttgc ggccgcactc
      781 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      841 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      901 ttgga
//
