LOCUS       sequence-31340-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EIF5A
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             538..555
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(608..626)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      622..669
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggctca gccaccttcc caatgcagtg
      121 ctcagcatta cgtaagaatg gctttgtggt gctcaaaggc cggccatgta agatcgtcga
      181 gatgtctact tcgaagactg gcaagcacgg ccacgccaag gtccatctgg ttggtattga
      241 catctttact gggaagaaat atgaagatat ctgcccgtca actcataata tggatgtccc
      301 caacatcaaa aggaatgact tccagctgat tggcatccag gatgggtacc tatcactgct
      361 ccaggacagc ggggaggtac gagaggacct tcgtctccct gagggagacc ttggcaagga
      421 gattgagcag aagtacgact gtggagaaga gatcctgatc acggtgctgt ctgccatgac
      481 agaggaggca gctgttgcaa tcaaggcctg atgacgaagc ttgcggccgc actcgagcac
      541 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      601 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      661 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      781 ccctagcgcc cgctccnttt cgctttcttc ccttcctttc tcgccccggt tcgccggctt
      841 tccccgtcaa gctctaaatc
//
