LOCUS       sequence-313401-        3393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses KCNH8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3393)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNH8_OE_1
FEATURES             Location/Qualifiers
     source          1..3393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(3323..3347)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3349..3390
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgccggtta tgaaaggatt actggcgccg caaaacacct tcctggacac catcgccacc
       61 cgttttgacg gaacacatag caacttcatc cttgccaatg cccaggtggc taagggtttc
      121 cccatagtct actgttccga tggcttctgc gagcttgctg gatttgcccg aactgaagtc
      181 atgcagaaga gttgtagctg caagttctta tttggggttg aaaccaatga gcaactgatg
      241 cttcaaatag aaaagtcact ggaggagaaa acagaattca aaggagaaat tatgttctac
      301 aagaaaaacg ggtctccatt ttggtgccta ctggatattg ttcccataaa gaatgaaaaa
      361 ggagatgtag tactttttct ggcctcgttc aaagatataa cagatacaaa agtgaagatt
      421 actccagaag ataaaaaaga agacaaagtc aaaggaagat caagagcagg gacccacttt
      481 gactcagccc ggagacggag tcgagcagtc ctttatcaca tctctgggca cctgcaaaga
      541 agagaaaaga acaaattgaa aataaataac aatgtttttg tagataaacc agcatttccg
      601 gagtataaag tttctgatgc aaaaaagtcc aaattcatac ttctgcattt tagcactttt
      661 aaagctggct gggactggct tattttgttg gcaacgtttt atgttgctgt gactgtacct
      721 tacaacgttt gctttattgg caatgacgac ctgtccacaa ctcggagcac aaccgtcagt
      781 gacattgcag tggagattct ttttattata gatattattt taaatttccg aacaacttat
      841 gtcagcaagt ctggccaagt tatctttgaa gcaagatcaa tttgcatcca ctatgtcaca
      901 acctggttca tcattgattt aatcgctgcc ctgccttttg atcttctgta tgctttcaac
      961 gtcacagtgg tgtctctcgt gcatcttcta aagacagtgc gcctcttgcg tcttttgcgt
     1021 ctgctgcaga agttagaccg ctattcccaa cacagtacta tcgtcctgac tctgctcatg
     1081 tccatgtttg cactccttgc acactggatg gcgtgtatct ggtacgtcat tggaaaaatg
     1141 gagagggaag acaacagcct tctgaagtgg gaagttggtt ggcttcatga gttgggaaag
     1201 agactggaat ctccatacta tggcaacaat accttggggg gcccgtcgat ccgaagtgcc
     1261 tatattgccg ctctgtactt cacgctgagc agcctcacca gcgtgggttt tgggaacgtc
     1321 tctgctaata cagatgcaga aaagatcttc tccatctgca ccatgctgat tggtgccttg
     1381 atgcacgcct tggtgtttgg aaacgtgaca gcaatcatac agaggatgta ctccagatgg
     1441 tccctctatc acactagaac taaggatctg aaagatttca tccgtgtcca tcacttgccc
     1501 caacaactca agcagaggat gctcgaatat tttcaaacaa cctggtcagt caacaatgga
     1561 atagattcaa atgagctttt gaaagacttt ccagatgaac tgcgttctga catcactatg
     1621 cacttgaaca aggagatctt acagttgtcc ctttttgaat gtgccagccg gggctgcctc
     1681 aggtctctgt ctctacacat caaaacctct ttctgtgctc cgggggagta tctgctgcgt
     1741 caaggggatg ctttgcaggc catctacttt gtatgctcgg gctccatgga agttcttaaa
     1801 gacagcatgg tgctggctat tcttgggaaa ggggatttaa ttggagcaaa tctatcaatt
     1861 aaggaccaag tgatcaagac caatgcagat gtaaaggctt taacctactg tgatctccag
     1921 tgtatcatcc tcaaaggact ctttgaagtg ctagaccttt acccagaata tgctcacaaa
     1981 ttcgtggaag acattcagca tgacctcaca tacaacctcc gagaaggtca tgagagtgat
     2041 gtgatatcaa gactatcaaa caaatctatg gtctcacagt cagagcccaa gggaaatggc
     2101 aacatcaaca agcgactccc atccattgtg gaagatgagg aagaggagga ggagggggag
     2161 gaagaggagg cagtctccct ctctcccatc tgcacaaggg gatcttcttc gcgcaacaag
     2221 aaggttggaa gcaataaagc ctacctgggc ttaagcttaa agcaactggc ctcgggaacg
     2281 gtgccctttc actcgcctat cagagtctcc aggtcaaatt cccccaaaac caagcaggaa
     2341 attgaccccc ccaaccataa taaaaggaaa gagaagaact tgaaattgca actttcaact
     2401 ttgaataatg ctggaccccc agacctcagt ccaaggattg ttgatggaat tgaagatgga
     2461 aacagcagtg aagaaagtca gacttttgat tttggctctg aacgaatcag atcagagccc
     2521 agaatttctc ctcctcttgg agatccagag attggagctg ctgttctctt catcaaagca
     2581 gaggagacca agcagcagat aaacaaactc aacagtgagg taacaacatt gactcaggaa
     2641 gtttctcagt tgggtaaaga catgagaaat gtgatccagc ttctggaaaa cgttctgtca
     2701 cctcagcagc catcacggtt ttgctctttg cacagcacct ctgtgtgtcc ctccagggag
     2761 agcttacaga ccagaacgag ctggagtgca caccagcctt gcctacactt gcaaacaggc
     2821 ggggctgctt atacccaagc acaactttgt agcagtaata tcacctcaga catttggagt
     2881 gtggatccct cctctgtggg gagcagcccc caacgaactg gagctcatga gcaaaatcct
     2941 gcagacagtg aactttatca ttctccaagc cttgattatt caccttccca ctaccaggtt
     3001 gtccaagaag gtcatttgca atttttaagg tgcatctctc cacattcaga ttctacgttg
     3061 acgcctctgc agtccatttc agcaactctc tcatcttctg tctgctcctc ttcggaaaca
     3121 tctttgcacc tagttctccc aagcagatca gaggagggca gcttcagtca gggaactgtg
     3181 agttccttca gtctggaaaa cttaccagga tcttggaacc aggaaggaat ggcatcagct
     3241 tctacaaaac ctttggagaa ccttccactg gaagttgtca caagcacagc agaagtgaaa
     3301 gataacaaag ccataaatgt atacccagct ttcttgtaca aagtggttgg taagcctatc
     3361 cctaaccctc tcctcggtct cgattctacg tag
//
