LOCUS       sequence-313428-        5241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus protein expression of KRAS4b (1-169).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Dharmaiah S, Tran TH, Messing S, Agamasu C, Gillette WK, Yan W, 
            Waybright T, Alexander P, Esposito D, Nissley DV, McCormick F, 
            Stephen AG, Simanshu DK
  TITLE     Structures of N-terminally processed KRAS provide insight into the 
            role of N-acetylation.
  JOURNAL   Sci Rep. 2019 Jul 19;9(1):10512. doi: 10.1038/s41598-019-46846-w.
  PUBMED    31324887
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-07-19"; volume: "9(1):10512. doi"; pages: " 
            10.1038/s41598-019-46846-w"
COMMENT     Sequence Label: R979-X57-008: Hs.KRAS4b(1-169)
FEATURES             Location/Qualifiers
     source          1..5241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..115
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     40..57
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    851..985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(888..907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     942..961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1014..1179
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1363..1817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1450..1469)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1659..1680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1843..1947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1948..2016,2017..2808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2166..2185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2979..3567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3468..3487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3721..3738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3872..4096
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4163..4696)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4885..4890,4891..4907,4908..4913))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
       61 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
      121 ggattattca taccgtccca ccatcgggcg cggatcatca caagtttgta caaaaaagtt
      181 ggcaccatga ctgaatataa acttgtggta gttggagctg gtggcgtagg caagagtgcc
      241 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      301 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      361 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      421 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      481 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      541 gatttgcctt ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      601 attcctttta ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca
      661 ttagttcgag aaattcgaaa acataaagaa aagtaatcaa ctttcttgta caaagtggtg
      721 atagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
      781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      961 caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga
     1021 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
     1081 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
     1141 tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt
     1201 tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt
     1261 tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg
     1321 actgaatatc aacgcttatt tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     1381 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1441 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1501 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1561 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     1621 tttttcgccc tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg
     1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1801 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1861 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1921 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1981 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2041 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2101 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2161 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2221 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2281 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2341 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2401 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2461 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2521 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2581 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2641 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2701 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2761 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2821 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2881 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3121 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3661 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3721 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     3781 ctgtgcggta tttcacaccg catagaccag ccgcgtaacc tggcaaaatc ggttacggtt
     3841 gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga
     3901 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
     3961 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
     4021 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat
     4081 ggtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc
     4141 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
     4201 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
     4261 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga
     4321 gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga
     4381 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
     4441 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
     4501 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
     4561 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
     4621 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
     4681 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     4741 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     4801 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     4861 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     4921 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     4981 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     5041 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     5101 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     5161 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     5221 tatagttcta gtggttggct a
//
