LOCUS       sequence-31343-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NET1
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             768..785
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(838..856)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggcg acatcaccat gaaggagtct
      121 ctcaccacca gggagatcag acggcaggag gcaatatatg aaatgtcccg aggtgaacag
      181 gatttaattg aggatctcaa acttgcaaga aaggcctacc atgaccccat gttaaagttg
      241 tccatcatgt cagaagagga actcacacat atatttggtg atctggactc ttacatacct
      301 ctgcatgaag atttgttgac aagaatagga gaagcaacca agcctgatgg aacagtggag
      361 cagattggtc acattctcgt gagctggtta ccgcgcttga atgcctacag aggttactgt
      421 agtaaccagc tggcagccaa agctcttctt gatcaaaaga aacaggatcc aagagtccaa
      481 gacttcctcc agcgatgtct cgagtctccc ttcagtcgaa aactagatct ttggagtttc
      541 ctagatatcc ctcgaagtcg cctagtcaaa taccctttac tgttaaaaga aattcttaaa
      601 cacactccaa aagagcaccc tgatgttcag cttctggagg atgctatatt gataatacag
      661 ggagtcctct ctgatatcaa cttgaagaaa ggtgaatccg agtgccagta ttacatcgac
      721 aagctggagt acctggattg atgacgaagc ttgcggccgc actcgagcac caccaccacc
      781 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      841 ctgagcaata actagcataa ccccttgggg cctctaaac
//
