LOCUS       sequence-313443-        6049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity 
            tag and a ybbR tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, 
            Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
  TITLE     Structural and functional insight into regulation of kinesin-1 by 
            microtubule-associated protein MAP7.
  JOURNAL   Science. 2022 Jan 21;375(6578):326-331. doi: 
            10.1126/science.abf6154. Epub 2022 Jan 20.
  PUBMED    35050657
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf6154"; journalName: "Science"; date: 
            "2022-01-21- 21"; volume: "375"; issue: "6578"; pages: "326-331"
COMMENT     Sequence Label: pOmnibac_ZZ_linker_ybbR_MAP7_N
FEATURES             Location/Qualifiers
     source          1..6049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  29..252
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(319..852)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1041..1046,1047..1063,1064..1069))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1140..1173)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1250..1341
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1266..1283
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1408..1431
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1474..1647
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1648..1821
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             1879..1899
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1921..1941
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3056..3190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3093..3112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3147..3166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3311..3476
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     3483..4188
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3571..3943
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3600..4188)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4378..4966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4867..4886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     5222..6049
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5222..5346
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5384..5989)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 gattgcgcag atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca
       61 aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa
      121 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
      181 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatgta
      241 aagactatat tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
      301 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc
      361 ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg
      421 cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc
      481 ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc
      541 actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc
      601 ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc
      661 ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc
      721 aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc
      781 catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
      841 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
      901 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
      961 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
     1021 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg
     1081 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgcca
     1141 taacttcgta tagcatacat tatacgaagt tatctgtaac tataacggtc ctaaggtagc
     1201 gagtttaaac actagtatcg attcgcgacc tactccggaa tattaataga tcatggagat
     1261 aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt
     1321 gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga
     1381 tctcggtccg aaaccatgtc gtactaccat caccatcacc atcaccatca cggttctatg
     1441 tgcatggcag gccttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa
     1501 caaaacgcgt actatgagat cttacattta cctaacttaa acgaaggaca acgaaacgcc
     1561 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
     1621 aagctaaatg atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac
     1681 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc
     1741 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc tgaaaagcta
     1801 aatgatgctc aggcgccgaa agtagacgca aattccgcgg ggaagtcaac cgattatgat
     1861 attccaacta ctgctagcga gaacctatat ttccagggag attatgatat ccctacgact
     1921 gagaatcttt attttcaggg catggactca ttggagttta tcgcatcaaa gctcgcagcg
     1981 gagctaggag ctggcggcga cggccacagg ggcggcgacg gcgcagtgcg aagcgaaaca
     2041 gcacccgaca gctacaaagt gcaagataag aaaaatgcct ccagccgccc tgcctctgca
     2101 atttcaggac aaaataacaa ccactcagga aataaaccag accctccgcc tgtgttacgt
     2161 gttgatgacc ggcagcggct ggcccgggag cgacgtgagg aacgggagaa acagctagct
     2221 gcaagagaaa tagtgtggtt agaaagagaa gagcgagcca ggcagcacta cgagaagcac
     2281 ctggaagagc ggaagaagag gttggaggag cagaggcaga aggaggagcg gaggagggct
     2341 gctgtggagg agaagcggag gcagagactt gaggaggaca aagaacgcca cgaagctgtt
     2401 gtacggcgca caatggaaag gagccagaag ccaaaacaga agcataaccg ttggtcgtgg
     2461 ggaggctctc tccatgggag ccctagcatc cacagtgcag atccagacag gcggtcagtt
     2521 tccaccatga atctttcgaa atatgttgat cccgtcatta gcaagcggct ctcctcttca
     2581 tctgcaactt tactaaattc tccagataga gctcgccgcc tgcagctcag cccatgggag
     2641 agcagcgttg ttaacagact cctgacgccc acacattcgt tcctggccag aagtaaaagc
     2701 acagctgcct tgtctggaga agcagcatct tgcagcccca tcatcatgcc ctacaaagct
     2761 gcacactcta gaaattcgat ggatcgacca aaactctttg taacaccacc tgagggctct
     2821 tctcgcagga ggatcattca tggcacagcg agctataaaa aagaaagaga gagagaaaat
     2881 gtactcttcc tcacatctgg cacccgaagg gctgtatctc cataatgagc ttgtcgagaa
     2941 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3001 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3181 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     3241 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta gggtataccc
     3301 atctaattgg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     3361 cgtattaact tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     3421 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacaaccg
     3481 gtggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac
     3541 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat
     3601 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     3661 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     3721 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     3781 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     3841 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     3901 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     3961 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca
     4021 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg
     4081 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg
     4141 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattac cggttgactt
     4201 gggtcaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4501 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5041 atcccctgat tgacttgggt cgctcttcct gtggatgcgc aggtatgtac aggaagaggt
     5101 ttatactaaa ctgttacatt gcaaacgtgg tttcgtgtgc caagtgtgaa aaccgatgtt
     5161 taatcaaggc tctgacgcat ttctacaacc acgactctaa gtgtgtgggt gaagtcatgc
     5221 atgcatcttt taatcaaatc ccaagatgtg tataaaccac caaactgcca aaaaatgaaa
     5281 actgtcgaca agctctgtcc gtttgctggc aactgcaagg gtctcaatcc tatttgtaat
     5341 tattgaataa taaaacaatt ataaatgtca aatttgtttt ttattaacga tacaaaccaa
     5401 acgcaacaag aacatttgta gtattatcta taattgaaaa cgcgtagtta taatcgctga
     5461 ggtaatattt aaaatcattt tcaaatgatt cacagttaat ttgcgacaat ataattttat
     5521 tttcacataa actagacgcc ttgtcgtctt cttcttcgta ttccttctct ttttcatttt
     5581 tctcttcata aaaattaaca tagttattat cgtatccata tatgtatcta tcgtatagag
     5641 taaatttttt gttgtcataa atatatatgt cttttttaat ggggtgtata gtaccgctgc
     5701 gcatagtttt tctgtaattt acaacagtgc tattttctgg tagttcttcg gagtgtgttg
     5761 ctttaattat taaatttata taatcaatga atttgggatc gtcggttttg tacaatatgt
     5821 tgccggcata gtacgcagct tcttctagtt caattacacc attttttagc agcaccggat
     5881 taacataact ttccaaaatg ttgtacgaac cgttaaacaa aaacagttca cctccctttt
     5941 ctatactatt gtctgcgagc agttgtttgt tgttaaaaat aacagccatt gtaatgagac
     6001 gcacaaacta atatcacaaa ctggaaatgt ctatcaatat atagttgct
//
