LOCUS       sequence-31345-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SETMAR (3F2K)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             805..822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(875..893)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggctgg gtgcctcatg agctgactga
      121 aaatcaaaaa aatcgtcgtt ttgaagtgtc atcttctctt attctacgca accacaacga
      181 accatttctc gatcggattg tgacgtgtga tgaaaagtgg attttatatg acaaccggcg
      241 acgatcagct cagtggttgg atcaagaaga agctccaaag cacttcccaa agccaatctt
      301 gcacccaaaa aaggtcatgg tcactatttg gtggtctgct gctggtctga tccactacag
      361 ctttctgaat cccggtgaaa ccattacatc tgagaagtat gctcaggaaa tcgatgagat
      421 gaaccaaaaa ctgcaacgcc tgcagctggc attggtcaac agaaagggcc caattcttct
      481 ccacgacaat gcccgaccgc atgttgcaca acccacactt caaaagttga atgaattggg
      541 ctatgaagtt ttgcctcatc caccgtattc acctgacctc ttgccaacca actaccacgt
      601 ctttaagcat ctcaacaact ttttgcaggg aaaacgcttc cacaaccagc aggatgcaga
      661 aaatgctttc caagagttcg tcgaatccca aagcacggat ttttacgcta caggaataaa
      721 ccaacttatt tctcgttggc aaaaatgtgt tgattgtaat ggttcctatt ttgattgatg
      781 acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      841 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      901 cttggggcct ctaaacgggt ctgga
//
