LOCUS       sequence-31346-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HDGF2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             407..424
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(477..495)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      491..538
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(662..681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcat gccacacgcc ttcaagcccg
      121 gggacttggt gttcgctaag atgaagggct accctcactg gcctgccagg atcgacgaca
      181 tcgcggatgg cgccgtgaag cccccaccca acaagtaccc catctttttc tttggcacac
      241 acgaaacagc cttcctggga cccaaggacc tgttccccta cgacaaatgt aaagacaagt
      301 acgggaagcc caacaagagg aaaggcttca atgaagggct gtgggagatc cagaacaacc
      361 cccacgccag ctacagctga tgacgaagct tgcggccgca ctcgagcacc accaccacca
      421 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      481 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      541 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      841 cctttgacgt tggagtccac gttctttaat agtggactct tg
//
