LOCUS       sequence-31347-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC80
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             346..363
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(416..434)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      430..477
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      514..872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(601..620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     811..832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagttc tctcatctct gcggtcagtg acaaactgag
      121 atggcggatg aaggaggaaa tggatcgtgc ccaggcagag ctcaatgcct tgaaacgaac
      181 agaagaagac ctgaaaaagg gtcaccagaa actggaagag atggttaccc gtttagatca
      241 agaagtagcc gaggttgata aaaacataga acttttgaaa aagaaggatg aagaactcag
      301 ttctgctctg gaaaaatgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac
      361 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      421 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      481 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      841 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccga
//
