LOCUS       sequence-31348-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RNF2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(530..548)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      544..591
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(715..734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcatg gatggtgcta gtgaaattga
      121 attagtattc aggcctcatc ccacacttat ggaaaaagat gacagtgcac agacgagata
      181 cataaagact tctggtaacg ccactgttga tcacttatcc aagtatctgg ctgtgaggtt
      241 agctttagaa gaacttcgaa gcaaaggtga atcaaaccag atgaaccttg atacagccag
      301 tgagaagcag tataccattt atatagcaac agccagtggc cagttcactg tattaaatgg
      361 ctctttttct ttggaattgg tcagtgagaa atactggaaa gtgaacaaac ccatggaact
      421 ttattacgca tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      481 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      541 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      601 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      841 attagggtga tggttcacgt agtgggccat cgccctgata gac
//
