LOCUS       sequence-313483-        7894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Melnykov A, Chen SJ, Varshavsky A
  TITLE     Gid10 as an alternative N-recognin of the Pro/N-degron pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15914-15923. doi: 
            10.1073/pnas.1908304116. Epub 2019 Jul 23.
  PUBMED    31337681
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1908304116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-08-6- 6"; volume: "116"; issue: "32"; pages: 
            "15914-15923"
COMMENT     Sequence Label: pAM1479a3
FEATURES             Location/Qualifiers
     source          1..7894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..597
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="VRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEG
                     KQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPE
                     LASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYP
                     GVLSEVQEEKGIKYKFEVYEKKD"
     protein_bind    complement(599..623)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             637..1077
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1088..1107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1088..1106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1126..1155
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      1195..1242
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1269..1456
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1324..1348)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1480..1496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1480..1496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1493..1515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1504..1520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1528..1534,1535..1552,1553..1558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1573..1594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1711..1728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1962..1981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2799..2846
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2871..2890
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3078..3872)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3078..3872)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3189..3208)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3799..3818
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3873..3977)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4004..5346
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4967..5014)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5387..5405
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5443..5465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5565..5584
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5605..5886
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5887..6561
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6664..7119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6801..6822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7013..7032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7190..7894
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 actagtatgt acattacggt tggtggagga gggggtggag ttcgaccatt gaactgcatc
       61 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
      121 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
      181 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
      241 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
      301 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
      361 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
      421 ccaggccacc tcagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
      481 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
      541 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaagacgac
      601 ccagctttct tgtacaaagt ggttgatggt accaagatga agctactgtc ttctatcgaa
      661 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      721 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      781 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      841 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      901 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      961 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
     1021 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
     1081 gaatttgtaa tacgactcac tatagggcga gccgccatca tggaggagca gaagctgatc
     1141 tcagaggagg acctgcatat ggccatggag gccgaatttg cagcggccgc ataactagca
     1201 taaccccttg gggcctctaa acgggtcttg aggggttttt tgcgcgcttg cagccaagct
     1261 aattccgggc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     1321 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     1381 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
     1441 accacacctc taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     2641 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     2701 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     2761 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     2821 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     2881 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     2941 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     3001 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     3061 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     3121 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     3181 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     3241 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     3301 gcatcgccat gagtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     3361 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     3421 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     3481 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     3541 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     3601 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     3661 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     3721 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     3781 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     3841 gaacctgcgt gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc
     3901 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3961 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct
     4021 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     4081 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
     4141 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     4201 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
     4261 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
     4321 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
     4381 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
     4441 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
     4501 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
     4561 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
     4621 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
     4681 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
     4741 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
     4801 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
     4861 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
     4921 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
     4981 ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     5041 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     5101 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     5161 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
     5221 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
     5281 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
     5341 tggatcatac taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5401 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5461 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5521 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     5581 tgtactgaga gtgcaccata gatcaacgac attactatat atataatata ggaagcattt
     5641 aatagaacag catcgtaata tatgtgtact ttgcagttat gacgccagat ggcagtagtg
     5701 gaagatattc tttattgaaa aatagcttgt caccttacgt acaatcttga tccggagctt
     5761 ttcttttttt gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag
     5821 ggagggcatt ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct
     5881 tggagtatgt ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc
     5941 ttgcagagca cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt
     6001 atatgtgtgc ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt
     6061 cttgtaaaag catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt
     6121 aatcaaccta aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa
     6181 ctgcatggag atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa
     6241 agactcgtat ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat
     6301 tcgtttattc ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac
     6361 tcgatttctg actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct
     6421 ggtggactga cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt
     6481 gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc
     6541 gtcaaaaatg ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc
     6601 acttgccgat ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     6661 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6721 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6781 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6841 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6901 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6961 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     7021 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     7081 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     7141 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt
     7201 gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat
     7261 tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata
     7321 gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca
     7381 ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc atcatcatat
     7441 cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa
     7501 cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg
     7561 acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga
     7621 tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt
     7681 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga
     7741 atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt
     7801 aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac
     7861 aatatttcaa gctataccaa gcatacaatc aact
//
