LOCUS       sequence-313489-        7390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato is expressed under the mycobacterial phsp60 heat shock 
            promoter for in vivo and in vitro detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Kong Y, Yang D, Cirillo SL, Li S, Akin A, Francis KP, Maloney T, 
            Cirillo JD
  TITLE     Application of Fluorescent Protein Expressing Strains to Evaluation 
            of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0149972. doi: 
            10.1371/journal.pone.0149972. eCollection 2016.
  PUBMED    26934495
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0149972. doi"; pages: " 
            10.1371/journal.pone.0149972. eCollection 2016"
COMMENT     Sequence Label: pJDC122
FEATURES             Location/Qualifiers
     source          1..7390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             580..609
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(613..621,622..636)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      643..652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             649..2079
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(2142..2160)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2156..2203
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2300..2755
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2387..2406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2597..2618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2781..2885
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3104..3123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5308..5896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5388..5407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7269..7315)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taacggccgc cagtgtgctg gaattcggct tttggtcgat accaagccat ttccgctgaa
       61 tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
      121 tagtgcctgt ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagacggt
      181 gaccacaacg cgcccgcttt gatcggggac gtctgcggcc gaccatttac gggtcttgtt
      241 gtcgttggcg gtcatgggcc gaacatactc acccggatcg gagggccgag gacaaggtcg
      301 aacgaggggc atgacccggt gcggggcttc ttgcactcgg cataggcgag tgctaagaat
      361 aacgttggca ctcgcgaccg gtgagtgcta ggtcgggacg gtgaggccag gcccgtcgtc
      421 gcagcgagtg gcagcgagga caacttgagc cgtccgtcgc gggcactgcg cccggccagc
      481 gtaagtagcg gggttgccgt cacccggtga cccccgtttc atccccgatc cggaggaatc
      541 acttcgcaat ggccaagaca attgcggatc cagctgcagg ctagcatgac tggtggacag
      601 caaatgggtc gggatctgta cgacgatgac gataaggatc ccgccaccat ggtgagcaag
      661 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
      721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      781 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
      841 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
      901 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
      961 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1021 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1081 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1141 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     1201 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     1261 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     1321 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     1381 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     1441 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1501 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1561 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     1621 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1681 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1741 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1801 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1861 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1921 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     1981 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2041 cacctgttcc tgtacggcat ggacgagctg tacaagtaag aattcgaagc ttgatccggc
     2101 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     2161 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     2221 atccggatct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2281 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2641 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2701 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3181 acttggttga gtactctgca gctggatcca tggatatcta gatttaaaga tctggtaccg
     3241 cggccgcgcc acggatgcca ccacaggcac tacaacggag ttcgccacgt acatcaccac
     3301 aaccaccgat tctggcggtg agctccccga tattcagcgg aaatggcttg gtatcgacca
     3361 agattcgtag aaccccgtct cgtctggctg gtattcaaaa cgggcgcaac gaaacacgca
     3421 acgagacagg catggcccaa accagaaaac tagcgtctac caggactttt acctgtccga
     3481 cccgttgcaa cggaaccccc cacggaaccc ccgcgacacc cgctccccaa ttgcgttaga
     3541 acagcggtgg attgtcggct tcgttgtggg ccttttgagc cgcttcctgt tctgccgcac
     3601 gctctttcct cgcccgatag ccgagtcgct taacggtgtc cagatgcagc ccgaaatgtt
     3661 tggccgtttg cggccaagag tggccctcgt cgtcgtgata ggcgcggatg cgttcgcggc
     3721 gtgcagcctg ctcggcgagc cactcgctgc gttcctgcgc cacgagccgg acgacgtggc
     3781 gttcggatag tccggtgatt cgagcgcctt cggcggcggt cacgcgccgc tttttgcgga
     3841 cagtcggctg ccggttgtag ccgtcgctgt agccgtcgct catagcaatg cctccatgca
     3901 tggctgacgc ggactttgcg cgccgcgcaa ctgtgctcgc cgccgtgcgc gctgctgcgc
     3961 ccttccgcga gatggccgac tggcgcgcac tgagtgtggc ctcgtagacc acgatcccgt
     4021 ccgcccaaat gcgcgacttg gttgtgatcc aacgccaaat gctgttggcg atggcgcgga
     4081 cctcgctgtc cggtagcggt ccgggacaca cgtcgttgca cggaaattcg gcgtttcgcg
     4141 cgtggcactc ggcatagatc gcgcggccga gtccgtccac gttccgggtc ggcaggtaga
     4201 tccgcatgag ggcgggacga taggcccaca acctgacgga atcgaacagt gcgcaattcc
     4261 gccctagcgg cgtcggagcc gctttgtacg tggtctgctg acgccagcgc ggcggtggca
     4321 tgttcgcgcc gagctcggcc tcgatgtggc tgagtgtgta gagatctgag tggagccatt
     4381 ccgtttccca ggcgatgtgg ccggggtttt tggtcatgag gcctgagtaa ctgcggtcgc
     4441 catcgacggc gcgccgaagg ccttcggcgc acgccgccat gtatgcgagc ggcttacgcc
     4501 gcgcgtattc ggtgcgtgga acaggggcgt tgagtgccca cactgcgtgt gcgtggccgt
     4561 tggcgcgatt gcccacgatc gcgttgggca gcggatggga cccccgggcg ctgagcgctc
     4621 ggagcgctgc gtctggatgg tctacgtcca cgaccagcag gtttgccagc gctgttgggt
     4681 tcgcctcgat gtaccggcgg cctagggccg acgcgcggct ttggcggtag atcccctcga
     4741 gcagatcgtc gcttgccagc ggccagtacg gcagccagag ctgctcaaat tcgtcggcga
     4801 cgtggctcac gcttggtagt agaccacgat taatcaccgg tgtatggtcc gacacgagct
     4861 ccaagtcaga tatttcgctg aggggccacc ccacaactgc acactccccc gctctcccgt
     4921 cgagccctgg tggtggaaca ccagcgacag ccgagcaccc ccaaccacct gtaccaacca
     4981 ggaggaacac atgcgtcgtt tcgaggacgt ttccgggccg ctgagagccg ctgtggcggc
     5041 cgtacacgcc gccttagacc cgttagaccc cctgccgcct gaatgcgcgg gtacgagcca
     5101 cacagcgccc gaacttacgg agctggtggg ctcacgcgtg cggccgcacg cgttgcgctc
     5161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5461 cctgtccgcc tttctcccct cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcatggaac
     5941 tagagcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6001 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6061 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6121 gacgtggccg accagcccgt catcgtcaac gcctgatccg cggtgcggac aggccgtgtc
     6181 gcgaccggcc gtgcggaatt aagccggccc gtaccctgtg aatagaggtc cgctgtgaca
     6241 caagaatccc tgttacttct cgaccgtatt gattcggatg attcctacgc gagcctgcgg
     6301 aacgaccagg aattctggga gccgctggcc cgccgagccc tggaggagct cgggctgccg
     6361 gtgccgccgg tgctgcgggt gcccggcgag agcaccaacc ccgtactggt cggcgagccc
     6421 ggcccggtga tcaagctgtt cggcgagcac tggtgcggtc cggagagcct cgcgtcggag
     6481 tcggaggcgt acgcggtcct ggcggacgcc ccggtgccgg tgccccgcct cctcggccgc
     6541 ggcgagctgc ggcccggcac cggagcctgg ccgtggccct acctggtgat gagccggatg
     6601 accggcacca cctggcggtc cgcgatggac ggcacgaccg accggaacgc gctgctcgcc
     6661 ctggcccgcg aactcggccg ggtgctcggc cggctgcaca gggtgccgct gaccgggaac
     6721 accgtgctca ccccccattc cgaggtcttc ccggaactgc tgcgggaacg ccgcgcggcg
     6781 accgtcgagg accaccgcgg gtggggctac ctctcgcccc ggctgctgga ccgcctggag
     6841 gactggctgc cggacgtgga cacgctgctg gccggccgcg aaccccggtt cgtccacggc
     6901 gacctgcacg ggaccaacat cttcgtggac ctggccgcga ccgaggtcac cgggatcgtc
     6961 gacttcaccg acgtctatgc gggagactcc cgctacagcc tggtgcaact gcatctcaac
     7021 gccttccggg gcgaccgcga gatcctggcc gcgctgctcg acggggcgca gtggaagcgg
     7081 accgaggact tcgcccgcga actgctcgcc ttcaccttcc tgcacgactt cgaggtgttc
     7141 gaggagaccc cgctggatct ctccggcttc accgatccgg aggaactggc gcagttcctc
     7201 tgggggccgc cggacaccgc ccccggcgcc tgacgccccc tctagctgat caccgcggcc
     7261 atgatggcat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     7321 tggcagtcga tcgtacgcta gttaactacg tcgacatcga taagcttggt accgagctcg
     7381 gatccactag
//
