LOCUS       sequence-313490-        6670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry is expressed under the mycobacterial phsp60 heat shock 
            promoter for in vivo and in vitro detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Kong Y, Yang D, Cirillo SL, Li S, Akin A, Francis KP, Maloney T, 
            Cirillo JD
  TITLE     Application of Fluorescent Protein Expressing Strains to Evaluation 
            of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0149972. doi: 
            10.1371/journal.pone.0149972. eCollection 2016.
  PUBMED    26934495
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0149972. doi"; pages: " 
            10.1371/journal.pone.0149972. eCollection 2016"
COMMENT     Sequence Label: pJDC124
FEATURES             Location/Qualifiers
     source          1..6670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             588..617
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(621..629,630..644)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             657..1367
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(802..820)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1070..1089
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1430..1448)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1444..1491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1588..2043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1675..1694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1885..1906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2069..2173
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2392..2411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4596..5184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4676..4695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6557..6603)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tccactagta acggccgcca gtgtgctgga attcggcttt tggtcgatac caagccattt
       61 ccgctgaata tcggggagct caccgccaga atcggtggtt gtggtgatgt acgtggcgaa
      121 ctccgttgta gtgcctgtgg tggcatccgt ggcgcggccg cggtaccaga tctttaaatc
      181 tagacggtga ccacaacgcg cccgctttga tcggggacgt ctgcggccga ccatttacgg
      241 gtcttgttgt cgttggcggt catgggccga acatactcac ccggatcgga gggccgagga
      301 caaggtcgaa cgaggggcat gacccggtgc ggggcttctt gcactcggca taggcgagtg
      361 ctaagaataa cgttggcact cgcgaccggt gagtgctagg tcgggacggt gaggccaggc
      421 ccgtcgtcgc agcgagtggc agcgaggaca acttgagccg tccgtcgcgg gcactgcgcc
      481 cggccagcgt aagtagcggg gttgccgtca cccggtgacc cccgtttcat ccccgatccg
      541 gaggaatcac ttcgcaatgg ccaagacaat tgcggatcca gctgcaggct agcatgactg
      601 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatccc gccaccatgg
      661 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      721 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      781 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      841 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      901 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      961 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     1021 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1081 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1141 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1201 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     1261 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1321 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagaa ttcgaagctt
     1381 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1441 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     1501 ggaactatat ccggatctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1561 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1921 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     2041 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2101 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2161 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2221 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2281 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     2341 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2401 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     2461 tcagaatgac ttggttgagt actctgcagc tggatccatg gatatctaga tttaaagatc
     2521 tggtaccgcg gccgcgccac ggatgccacc acaggcacta caacggagtt cgccacgtac
     2581 atcaccacaa ccaccgattc tggcggtgag ctccccgata ttcagcggaa atggcttggt
     2641 atcgaccaag attcgtagaa ccccgtctcg tctggctggt attcaaaacg ggcgcaacga
     2701 aacacgcaac gagacaggca tggcccaaac cagaaaacta gcgtctacca ggacttttac
     2761 ctgtccgacc cgttgcaacg gaacccccca cggaaccccc gcgacacccg ctccccaatt
     2821 gcgttagaac agcggtggat tgtcggcttc gttgtgggcc ttttgagccg cttcctgttc
     2881 tgccgcacgc tctttcctcg cccgatagcc gagtcgctta acggtgtcca gatgcagccc
     2941 gaaatgtttg gccgtttgcg gccaagagtg gccctcgtcg tcgtgatagg cgcggatgcg
     3001 ttcgcggcgt gcagcctgct cggcgagcca ctcgctgcgt tcctgcgcca cgagccggac
     3061 gacgtggcgt tcggatagtc cggtgattcg agcgccttcg gcggcggtca cgcgccgctt
     3121 tttgcggaca gtcggctgcc ggttgtagcc gtcgctgtag ccgtcgctca tagcaatgcc
     3181 tccatgcatg gctgacgcgg actttgcgcg ccgcgcaact gtgctcgccg ccgtgcgcgc
     3241 tgctgcgccc ttccgcgaga tggccgactg gcgcgcactg agtgtggcct cgtagaccac
     3301 gatcccgtcc gcccaaatgc gcgacttggt tgtgatccaa cgccaaatgc tgttggcgat
     3361 ggcgcggacc tcgctgtccg gtagcggtcc gggacacacg tcgttgcacg gaaattcggc
     3421 gtttcgcgcg tggcactcgg catagatcgc gcggccgagt ccgtccacgt tccgggtcgg
     3481 caggtagatc cgcatgaggg cgggacgata ggcccacaac ctgacggaat cgaacagtgc
     3541 gcaattccgc cctagcggcg tcggagccgc tttgtacgtg gtctgctgac gccagcgcgg
     3601 cggtggcatg ttcgcgccga gctcggcctc gatgtggctg agtgtgtaga gatctgagtg
     3661 gagccattcc gtttcccagg cgatgtggcc ggggtttttg gtcatgaggc ctgagtaact
     3721 gcggtcgcca tcgacggcgc gccgaaggcc ttcggcgcac gccgccatgt atgcgagcgg
     3781 cttacgccgc gcgtattcgg tgcgtggaac aggggcgttg agtgcccaca ctgcgtgtgc
     3841 gtggccgttg gcgcgattgc ccacgatcgc gttgggcagc ggatgggacc cccgggcgct
     3901 gagcgctcgg agcgctgcgt ctggatggtc tacgtccacg accagcaggt ttgccagcgc
     3961 tgttgggttc gcctcgatgt accggcggcc tagggccgac gcgcggcttt ggcggtagat
     4021 cccctcgagc agatcgtcgc ttgccagcgg ccagtacggc agccagagct gctcaaattc
     4081 gtcggcgacg tggctcacgc ttggtagtag accacgatta atcaccggtg tatggtccga
     4141 cacgagctcc aagtcagata tttcgctgag gggccacccc acaactgcac actcccccgc
     4201 tctcccgtcg agccctggtg gtggaacacc agcgacagcc gagcaccccc aaccacctgt
     4261 accaaccagg aggaacacat gcgtcgtttc gaggacgttt ccgggccgct gagagccgct
     4321 gtggcggccg tacacgccgc cttagacccg ttagaccccc tgccgcctga atgcgcgggt
     4381 acgagccaca cagcgcccga acttacggag ctggtgggct cacgcgtgcg gccgcacgcg
     4441 ttgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4741 accggatacc tgtccgcctt tctcccctcg ggaagcgtgg cgctttctca tagctcacgc
     4801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5221 catggaacta gagcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5401 gactccccga cgtggccgac cagcccgtca tcgtcaacgc ctgatccgcg gtgcggacag
     5461 gccgtgtcgc gaccggccgt gcggaattaa gccggcccgt accctgtgaa tagaggtccg
     5521 ctgtgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
     5581 gcctgcggaa cgaccaggaa ttctgggagc cgctggcccg ccgagccctg gaggagctcg
     5641 ggctgccggt gccgccggtg ctgcgggtgc ccggcgagag caccaacccc gtactggtcg
     5701 gcgagcccgg cccggtgatc aagctgttcg gcgagcactg gtgcggtccg gagagcctcg
     5761 cgtcggagtc ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg ccccgcctcc
     5821 tcggccgcgg cgagctgcgg cccggcaccg gagcctggcc gtggccctac ctggtgatga
     5881 gccggatgac cggcaccacc tggcggtccg cgatggacgg cacgaccgac cggaacgcgc
     5941 tgctcgccct ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg gtgccgctga
     6001 ccgggaacac cgtgctcacc ccccattccg aggtcttccc ggaactgctg cgggaacgcc
     6061 gcgcggcgac cgtcgaggac caccgcgggt ggggctacct ctcgccccgg ctgctggacc
     6121 gcctggagga ctggctgccg gacgtggaca cgctgctggc cggccgcgaa ccccggttcg
     6181 tccacggcga cctgcacggg accaacatct tcgtggacct ggccgcgacc gaggtcaccg
     6241 ggatcgtcga cttcaccgac gtctatgcgg gagactcccg ctacagcctg gtgcaactgc
     6301 atctcaacgc cttccggggc gaccgcgaga tcctggccgc gctgctcgac ggggcgcagt
     6361 ggaagcggac cgaggacttc gcccgcgaac tgctcgcctt caccttcctg cacgacttcg
     6421 aggtgttcga ggagaccccg ctggatctct ccggcttcac cgatccggag gaactggcgc
     6481 agttcctctg ggggccgccg gacaccgccc ccggcgcctg acgccccctc tagctgatca
     6541 ccgcggccat gatggcataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     6601 ttgatgcctg gcagtcgatc gtacgctagt taactacgtc gacatcgata agcttggtac
     6661 cgagctcgga
//
