LOCUS       sequence-31351-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK3 (3K21)
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(686..704)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      700..747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgatatttt gtttacttta gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcat ccatgttctg gaaaacttta aaaattatgg
      121 actgctgctg aagtttcaaa aactggctat gacgattatt gcgcaacaat cgaatgatta
      181 tgatgttgaa aaactgaaaa gtacctttct ggtgctggat gaagatggaa aaggttatat
      241 caccaaagag cagctgaaaa agggcctgga aaaagatggg ctgaaactgc cgtacaattt
      301 tgacctgctg ctggatcaga tcgatagcga tggttccggt aaaattgact atacggaatt
      361 tattgcggcg gcactggacc gcaaacaact gtctaaaaaa ctgatttact gtgcgttccg
      421 tgtgtttgac gtcgataatg atggagaaat tacgaccgca gaactggccc atattctgta
      481 taatggtaac aaaaagggca acattaccca gcgcgatgtg aatcgtgtta aacgcatgat
      541 tcgtgatgtt gacaagaaca acgatggcaa aatcgatttt catgaattca gtgaaatgat
      601 gaaactgtga tgacgaagct tgcggccgca ctcgaggatc cggctgctaa caaagcccga
      661 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      721 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca
      781 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
      841 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
      901 ttaactgtga taaacctacc gcattaaagc ttatcgatga taagctgtc
//
