LOCUS       sequence-313512-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti-viral construct with Cre recombinase and doxycycline inducible
            expression of tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Loganathan SK, Schleicher K, Malik A, Quevedo R, Langille E, Teng K,
            Oh RH, Rathod B, Tsai R, Samavarchi-Tehrani P, Pugh TJ, Gingras AC, 
            Schramek D
  TITLE     Rare driver mutations in head and neck squamous cell carcinomas 
            converge on NOTCH signaling.
  JOURNAL   Science. 2020 Mar 13;367(6483):1264-1269. doi: 
            10.1126/science.aax0902.
  PUBMED    32165588
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-03-13- 13"; volume: "367"; 
            issue: "6483"; pages: "1264-1269"
COMMENT     Sequence Label: PLX306 iCre tetO tomato rtTA
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             543..1970
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIHKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTGTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(1971..1997)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2008..2025
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2035..2545
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2464..2482
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3602..3658
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3659..4276,4277..4282,4283..4318,4319..4321,4322..4357,
                     4358..4360,4361..4396,4397..4405)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4445..4465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4863..4874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5079..5312
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5384..5505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5421..5440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5475..5494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5545..5680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5680..5751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5752..5820,5821..6612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5970..5989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6783..7371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7272..7291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7525..7542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7659..7680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7695..7700,7701..7718,7719..7725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7733..7749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7738..7760
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7757..7773
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7757..7773
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7792..7812
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7794..7812
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7840..8066
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8067..8247
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8294..8419
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8912..9145
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9330..9374
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
      601 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      661 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
      721 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
      781 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      841 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
      901 tccacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
      961 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     1021 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     1081 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     1141 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     1201 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     1261 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     1321 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1381 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     1441 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     1501 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1561 tgaacttcga ggacggcggt ctggtgaccg ggacccagga ctcctccctg caggacggca
     1621 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     1681 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     1741 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     1801 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     1861 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     1921 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag agcgtaatct
     1981 ggaacatcgt atgggtatag accggtccac caccaccacc accactaagg atccggggtt
     2041 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     2101 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     2161 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     2221 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     2281 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     2341 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     2401 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     2461 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     2521 ccgaatcacc gacctctctc cccagggttt aaacatggtc tccaacctgc tgactgtgca
     2581 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
     2641 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     2701 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
     2761 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
     2821 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
     2881 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
     2941 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
     3001 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
     3061 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
     3121 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     3181 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     3241 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     3301 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     3361 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     3421 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     3481 aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct
     3541 ggactctgag actggggcca tggtgaggct tctagaggat ggggatggct ccggagaatt
     3601 cgccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcccat
     3661 gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     3721 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     3781 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     3841 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     3901 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     3961 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     4021 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     4081 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     4141 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     4201 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     4261 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     4321 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     4381 tgaccttgac atgctccccg ggtaactaag taaggatcga tccaagatat cgtattctta
     4441 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4501 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4561 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4621 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4681 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4741 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4801 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4861 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4921 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4981 ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt acaaggcagc
     5041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5101 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5281 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5341 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5521 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5641 gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat gtatccgctc
     5701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6901 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7441 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7501 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7561 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7621 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7681 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7741 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     7801 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     7861 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     7921 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     7981 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     8041 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     8101 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8161 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8221 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     8281 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     8341 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     8401 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     8461 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     8521 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     8581 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     8641 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     8701 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     8761 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     8821 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     8881 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     8941 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9001 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9061 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9121 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9181 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     9241 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9301 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9361 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9421 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     9481 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     9541 ggggac
//
