LOCUS       sequence-313513-        7567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, 
            Brugge JS, Swat W
  TITLE     Vav family proteins couple to diverse cell surface receptors.
  JOURNAL   Mol Cell Biol. 2000 Sep . 20(17):6364-73.
  PUBMED    10938113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Sep . 20(17):6364-73."
COMMENT     Sequence Label: pC.HA Vav1
FEATURES             Location/Qualifiers
     source          1..7567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             3208..3234
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          3266..3838
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3970..3989)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4035..4090
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4089..4108)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4444..4579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4685..4702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4856..5444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4936..4955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5615..6406,6407..6475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6238..6257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6476..6580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6648..6666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6704..6726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6826..6845
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6912..7367)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7049..7070)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7261..7280
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7493..7515
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7507..7524
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7508..7524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7531..7550
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7531..7549
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      661 tctagaatgg agctgtggcg ccaatgcacc cactggctca tccagtgccg ggtgctgccg
      721 cccagccacc gcgtgacctg ggatggggct caggtgtgtg aactggccca ggccctccgg
      781 gatggtgtcc ttctgtgtca gctgcttaac aacctgctac cccatgccat caacctgcgt
      841 gaggtcaacc tgcgccccca gatgtcccag ttcctgtgcc ttaagaacat tagaaccttc
      901 ctgtccacct gctgtgagaa gttcggcctc aagcggagcg agctcttcga agcctttgac
      961 ctcttcgatg tgcaggattt tggcaaggtc atctacaccc tgtctgctct gtcctggacc
     1021 ccgatcgccc agaacagggg gatcatgccc ttccccaccg aggaggagag tgtaggtgat
     1081 gaagacatct acagtggcct gtccgaccag atcgacgaca cggtggagga ggatgaggac
     1141 ctgtatgact gcgtggagaa tgaggaggcg gaaggcgacg agatctatga ggacctcatg
     1201 cgctcggagc ccgtgtccat gccgcccaag atgacagagt atgacaagcg ctgctgctgc
     1261 ctgcgggaga tccagcagac ggaggagaag tacactgaca cgctgggctc catccagcag
     1321 catttcttga agcccctgca acggttcctg aaacctcaag acattgagat catctttatc
     1381 aacattgagg acctgcttcg tgttcatact cacttcctaa aggagatgaa ggaagccctg
     1441 ggcacccctg gcgcagccaa tctctaccag gtcttcatca aatacaagga gaggttcctc
     1501 gtctatggcc gctactgcag ccaggtggag tcagccagca aacacctgga ccgtgtggcc
     1561 gcagcccggg aggacgtgca gatgaagctg gaggaatgtt ctcagagagc caacaacggg
     1621 aggttcaccc tgcgggacct gctgatggtg cctatgcagc gagttctcaa atatcacctc
     1681 cttctccagg agctggtgaa acacacgcag gaggcgatgg agaaggagaa cctgcggctg
     1741 gccctggatg ccatgaggga cctggctcag tgcgtgaacg aggtcaagcg agacaacgag
     1801 acactgcgac agatcaccaa tttccagctg tccattgaga acctggacca gtctctggct
     1861 cactatggcc ggcccaagat cgacggggaa ctcaagatca cctcggtgga acggcgctcc
     1921 aagatggaca ggtatgcctt cctgctcgac aaagctctac tcatctgtaa gcgcagggga
     1981 gactcctatg acctcaagga ctttgtaaac ctgcacagct tccaggttcg ggatgactct
     2041 tcaggagacc gagacaacaa gaagtggagc cacatgttcc tcctgatcga ggaccaaggt
     2101 gcccagggct atgagctgtt cttcaagaca agagaattga agaagaagtg gatggagcag
     2161 tttgagatgg ccatctccaa catctatccg gagaatgcca ccgccaacgg gcatgacttc
     2221 cagatgttct cctttgagga gaccacatcc tgcaaggcct gtcagatgct gcttagaggt
     2281 accttctatc agggctaccg ctgccatcgg tgccgggcat ctgcacacaa ggagtgtctg
     2341 gggagggtcc ctccatgtgg ccgacatggg caagatttcc caggaactat gaagaaggac
     2401 aaactacatc gcagggctca ggacaaaaag aggaatgagc tgggtctgcc caagatggag
     2461 gtgtttcagg aatactacgg gcttcctcca ccccctggag ccattggacc ctttctacgg
     2521 ctcaaccctg gagacattgt ggagctcacg aaggctgagg ctgaacagaa ctggtgggag
     2581 ggcagaaata catctactaa tgaaattggc tggtttcctt gtaacagggt gaagccctat
     2641 gtccatggcc ctcctcagga cctgtctgtt catctctggt acgcaggccc catggagcgg
     2701 gcaggggcag agagcatcct ggccaaccgc tcggacggga ctttcttggt gcggcagagg
     2761 gtgaaggatg cagcagaatt tgccatcagc attaaatata acgtcgaggt caagcacatt
     2821 aaaatcatga cagcagaagg actgtaccgg atcacagaga aaaaggcttt ccgggggctt
     2881 acggagctgg tggagtttta ccagcagaac tctctaaagg attgcttcaa gtctctggac
     2941 accaccttgc agttcccctt caaggagcct gaaaagagaa ccatcagcag gccagcagtg
     3001 ggaagcacaa agtattttgg cacagccaaa gcccgctatg acttctgcgc ccgagaccga
     3061 tcagagctgt cgctcaagga gggtgacatc atcaagatcc ttaacaagaa gggacagcaa
     3121 ggctggtggc gaggggagat ctatggccgg gttggctggt tccctgccaa ctacgtggag
     3181 gaagattatt ctgaatactg cactagttat ccgtacgacg taccagacta cgcataagaa
     3241 aagtgaggat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
     3301 cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat
     3361 gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct
     3421 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
     3481 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
     3541 taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc
     3601 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
     3661 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc tggtcatcat
     3721 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
     3781 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt tcctacagct
     3841 cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcactcctca
     3901 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     3961 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     4021 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     4081 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     4141 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     4201 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     4261 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     4321 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     4381 cccagtcata gctgtccctc ttctcttatg gagatccctc gaggagcttt ttgcaaaagc
     4441 ctaggcctcc aaaaaagcct cttcactact tctggaatag ctcagaggcc gaggcggcct
     4501 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     4561 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattgc
     4621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6601 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6661 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6721 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6781 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6841 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6901 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6961 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7021 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     7081 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     7141 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     7201 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     7261 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     7321 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     7381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7441 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7501 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattcgag
     7561 ctcgccc
//
