LOCUS       sequence-313514-        9907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Fzd4 subtype NGS Wnt in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Miao Y, Ha A, de Lau W, Yuki K, Santos AJM, You C, Geurts MH, 
            Puschhof J, Pleguezuelos-Manzano C, Peng WC, Senlice R, Piani C, 
            Buikema JW, Gbenedio OM, Vallon M, Yuan J, de Haan S, Hemrika W, 
            Rosch K, Dang LT, Baker D, Ott M, Depeille P, Wu SM, Drost J, Nusse 
            R, Roose JP, Piehler J, Boj SF, Janda CY, Clevers H, Kuo CJ, Garcia 
            KC
  TITLE     Next-Generation Surrogate Wnts Support Organoid Growth and 
            Deconvolute Frizzled Pleiotropy In Vivo.
  JOURNAL   Cell Stem Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020.
  PUBMED    32818433
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Aug 14. pii: S1934-5909(20)30358-1. doi: 
            10.1016/j.stem.2020.07.020."
COMMENT     Sequence Label: pHR-Fzd4 subtype NGS Wnt
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..89)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        74..403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      254..389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1537..1602
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1732..1752
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2177..2311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2214..2233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2268..2287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2479..3112
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3159..3284
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3781..4014
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4199..4243
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4528..4645
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4798..5205
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6169..6186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6249..6837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6302..6322)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6720..6731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6840..6856)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6841..6857)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6947..7180
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7205..7223)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7206..7223)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7513..7532)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7746..7765)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7865..7887
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7925..7943)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8011..8115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8116..8184,8185..8976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8334..8353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9147..9735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9636..9655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9889..9906
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
       61 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
      421 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
      481 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
      541 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
      601 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
      661 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
      721 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
      781 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
      841 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
      901 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
      961 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     1021 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     1081 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     1141 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     1201 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     1261 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     1321 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     1381 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     1441 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     1501 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     1561 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     1621 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     1681 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     1741 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     1801 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     1861 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     1921 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     1981 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     2041 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     2101 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2161 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2281 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     2341 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     2401 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     2461 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     2521 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     2581 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     2641 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     2701 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     2761 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     2821 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     2881 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     2941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     3121 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     3181 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     3241 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     3301 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     3361 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     3421 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     3481 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     3541 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     3601 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     3661 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     3721 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     3781 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     3841 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     3901 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     3961 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     4021 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     4081 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     4141 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     4201 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     4261 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     4321 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     4381 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     4441 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     4501 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4561 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4621 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     4681 gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat tttatttagt
     4741 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     4801 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     4861 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     4921 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     4981 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     5041 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     5101 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     5161 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga tctggagctc
     5221 tcgagaattc tcacgcgtgc caccatgaag acgatcatcg ccctgagcta catcttctgc
     5281 ctggtgttcg ccaaaatgta tcacactaag ggacaggaag gcagcgtgtg cctgaggtcc
     5341 tctgactgtg catctggcct gtgctgcgcc agacactttt ggtccaaaat ctgcaagccg
     5401 gttctgaaag agggccaggt gtgcacaaag catcgacgaa agggcagcca tggactcgag
     5461 atctttcaga ggtgctactg cggggagggt ctgtcttgca gaatccagaa agatcatcat
     5521 caggcctcca actcctcccg cctgcacacc tgccagcgac atggaagcgg ttccggaggt
     5581 tctggctccg gatctgaact gggtaaacgt ctgatccgtg cagcacttga cggtaacaaa
     5641 gaccgtgtta aagacctcat tgaaaatggt gctgacgtta acgcgtccct gatgtctggg
     5701 acgactccgt tatatgctgc cgccatgaac ggccacaaag aggttgtgaa gttactgatc
     5761 tccaagggcg cagatgtgaa tgctcagtcc gttgcgggtt ctacacctct ggttgcggcg
     5821 gcgaatttcg gacataacga agtggtaaaa ctgctgataa gtaaaggagc agacgtcaat
     5881 gctgtgactg cgtttggagt gaccccccta catgctgctg ctgctgatgg gcacaaggaa
     5941 gtagtgaagt tgcttatttc taagggggcc gacgtaaatg cgaaagctgg tcgtggcatg
     6001 actccactcc atattgcagc attccgcggt cataaggaag tcgttaaact attaatcagc
     6061 aaaggtgcgg atttgaacac ctctgccaaa gacggtgcaa ccccgcttga catggcgcgt
     6121 gaatctggca atgaggaggt tgtcaagctc ttggaaaagc aactcgagca ccaccaccac
     6181 caccactgag cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     6241 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6301 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6361 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6421 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6481 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6541 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6601 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6661 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6721 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6781 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     6841 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     6901 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6961 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7021 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7081 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7141 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     7201 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     7261 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     7321 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     7381 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     7441 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     7501 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     7561 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     7621 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     7681 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     7741 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7801 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7921 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8281 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8341 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8641 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9781 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9841 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9901 agcgagg
//
