LOCUS       sequence-31353-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NMT1 (3IU2)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     278..297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     381..400
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        381..399
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    400..424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             439..461
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             471..488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             501..521
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccaccctgat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
       61 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
      121 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
      181 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
      241 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
      301 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      361 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      421 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
      481 atcatcacag cagcggcaga gaaaacttgt atttccaggg ccgaagctac cagttctggg
      541 atacgcagcc cgtccccaag ctgggcgaag tggtgaacac ccatggcccc gtggagcctg
      601 acaaggacaa tatccgccag gagccctaca ccctgcccca gggcttcacc tgggatgctt
      661 tggacctggg cgatcgtggt gtgctaaaag aactgtacac cctcctgaat gagaactatg
      721 tggaagatga tgacaacatg ttccgatttg attattcccc ggagtttctt ttgtgggctc
      781 tccggccacc cggctggctc ccccagtggc actgtggggt tcgagtggtc tcaagtcgga
      841 aattggttgg gttcattagc gccatcccag caaacatcca tatctatgac aca
//
