LOCUS       sequence-313537-       17038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TcTT2  in Ti plasmid binary vector pGZ12.0501 (KF871320.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17038)
  AUTHORS   Florez SL, Erwin RL, Maximova SN, Guiltinan MJ, Curtis WR
  TITLE     Enhanced somatic embryogenesis in Theobroma cacao using the 
            homologous BABY BOOM transcription factor.
  JOURNAL   BMC Plant Biol. 2015 May 16;15:121. doi: 10.1186/s12870-015-0479-4.
  PUBMED    25976599
REFERENCE   2  (bases 1 to 17038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2015-05-16"; volume: "15:121. doi"; pages: " 
            10.1186/s12870-015-0479-4"
COMMENT     Sequence Label: pGZ12.0501
FEATURES             Location/Qualifiers
     source          1..17038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    390..414
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        538..717
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        570..753
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             774..1568
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      1958..2210
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3188..3233
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    3525..3536
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        5057..5102
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5202..5996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6200..6374
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7091..7136
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      7236..7245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7242..7244,7245..7247,7248..7961)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7242..7244,7245..7247,7248..7958)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7287..7308)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7548..7567)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7895..7916
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7999..8173
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8204..8221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8204..8220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8213..8235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8360..8379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8570..8591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8838..8862
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10458..11606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11905..12699)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12891..13658
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14281..14992
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15119..15490)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            15523..15632
                     /note="incP origin of transfer"
     primer_bind     15855..15874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16108..16125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(16313..16963)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 gaccagcgga ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt
       61 ctgagcactg tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc
      121 ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg
      181 ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc
      241 gggcggcggc caatcttgct cgtctcgctg gccggcgcca gatctgggga accctgtggt
      301 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
      361 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
      421 gatagtttaa actgaaggcg ggaaacgaca atctgatcat gagcggagaa ttaagggagt
      481 cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa
      541 ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat
      601 acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat
      661 atttcttgtc aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag
      721 tctcatattc actctcaatc caaataatct gcaccggatc tggatcgttt cgcatgattg
      781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
      841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
      901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
      961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     1021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     1081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     1141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     1201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     1261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg
     1321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1561 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     1621 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     1681 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccacgg
     1741 gatctctgcg gaacaggcgg tcgaaggtgc cgatatcatt acgacagcaa cggccgacaa
     1801 gcacaacgcc acgatcctga gcgacaatat gatcgggccc ggcgtccaca tcaacggcgt
     1861 cggcggcgac tgcccaggca agaccgagat gcaccgcgat atcttgctgc gttcggatat
     1921 tttcgtggag ttcccgccac agacccggat gatccccgat cgttcaaaca tttggcaata
     1981 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     2041 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     2101 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     2161 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggcctcctg
     2221 tcaatgctgg cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg
     2281 agggtggcgg ttctgagggt ggcggctctg agggaggcgg ttccggtggt ggctctggtt
     2341 ccggtgattt tgattatgaa aagatggcaa acgctaataa gggggctatg accgaaaatg
     2401 ccgatgaaaa cgcgctacag tctgacgcta aaggcaaact tgattctgtc gctactgatt
     2461 acggtgctgc tatcgatagg cctagcttgc caacatggtg gagcacgaca ctctcgtcta
     2521 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
     2581 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
     2641 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
     2701 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     2761 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca
     2821 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     2881 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     2941 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     3001 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     3061 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     3121 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     3181 atccttcgca agacccttcc tctatataag gaagttcatt tcattcggag aggtacgtat
     3241 ttttacaaca attaccaaca acaacaacaa acaacaacaa cattacattt tacattctac
     3301 aaactagtat ggggagggct ccttgctgct caaaagtggg gctgcacagg ggaccttgga
     3361 cacctaggga agacacactg ctcgtgaagt atatccaggc ccacggagat ggtcattgga
     3421 gaagcttgcc taagaaagct ggcctcctta gatgcgggaa gtcatgtaga ctcaggtgga
     3481 tgaactacct tagacctgac ataaaaaggg gcaatattac tccagatgag gatgacctca
     3541 ttatcagact tcacagcttg ctgggtaaca ggtggtcatt gatcgctggc agactgccag
     3601 ggaggactga caatgagatt aagaactatt ggaatacaca cctttcaaaa agactccttt
     3661 ctcagggaac tgatcctaac acacataaga aattgagcga accagcagtt cagcaagtga
     3721 agaaaagaaa gagctcaagg ggaaactcta ataagaagca aaacaatagc aagggcaaag
     3781 gggccaaggt tgagcctgaa aagccaaaag tgcatttgcc taaaccagtt agagtgactt
     3841 ctttcagcct gcctaggaac gactcattcg atcagtgcaa tactttttct acagtttcta
     3901 gctcacaagg aggagagggc gggctcggaa ctgaagttgt gcaaggtcca tggtctgata
     3961 acgttaatga tgacgagaac ggaacaggtt ttcttgctgc atacgatgac cacggattcg
     4021 ttaatggttc agattttgag tgtcagagcc atgtgcctgc atcagatgac gataattctt
     4081 tggaaaagct gtacgaggaa tatcttcaat tgctcaagac taacgacgac caggttcagt
     4141 tggactcatt tgccgaatca ttgctcatct aagttaactt tctccataat aatgtgtgag
     4201 tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata
     4261 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     4321 ctaaaaccaa aatccagtcc taggaatcga taagcttgcc aacatggtgg agcacgacac
     4381 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     4441 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     4501 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     4561 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     4621 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     4681 atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     4741 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     4801 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     4861 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     4921 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     4981 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     5041 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt cattcggaga
     5101 ggtacgtatt tttacaacaa ttaccaacaa caacaacaaa caacaacaac attacatttt
     5161 acattctaca actacatcta gaattccccg gatcgtttcg catgattgaa caagatggat
     5221 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5281 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5341 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag gcagcgcggc
     5401 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5461 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5521 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5581 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5641 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5701 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5761 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5821 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5881 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5941 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6001 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6061 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6121 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg gaattctaga
     6181 attggcatgc aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     6241 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     6301 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     6361 ctaaaatcca gatcccccga attccaagct tgccaacatg gtggagcacg acactctcgt
     6421 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     6481 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     6541 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     6601 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     6661 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     6721 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     6781 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     6841 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     6901 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
     6961 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     7021 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     7081 actatccttc gcaagaccct tcctctatat aaggaagttc atttcattcg gagaggtacg
     7141 tatttttaca acaattacca acaacaacaa caaacaacaa caacattaca ttttacattc
     7201 tacaactaca tctagaggat ccccgggtac cggtcgccac catggtgagc aagggcgagg
     7261 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7321 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7381 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7441 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7501 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7561 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7621 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7681 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     7741 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7801 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     7861 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7921 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagaa
     7981 ttggcatgca agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat
     8041 tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     8101 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
     8161 taaaatccag atcccccgaa ttccgggtac cgagctcgaa ttcactggcc gtcgttttac
     8221 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8281 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8341 gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8461 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     8521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8581 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     8641 ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     8701 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     8761 cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt gttattaagt
     8821 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     8881 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     8941 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     9001 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     9061 gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga
     9121 gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt
     9181 gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc
     9241 gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg ctcaccgaat
     9301 ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg gctgatcgac
     9361 cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     9421 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     9481 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     9541 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     9601 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     9661 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
     9721 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
     9781 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
     9841 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
     9901 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
     9961 taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg
    10021 ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt
    10081 gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg
    10141 caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa
    10201 catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc
    10261 cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat
    10321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
    10381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
    10441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
    10501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
    10561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
    10621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
    10681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
    10741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
    10801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
    10861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
    10921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
    10981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
    11041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
    11101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
    11161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
    11221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
    11281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
    11341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
    11401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
    11461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
    11521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
    11581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
    11641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
    11701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
    11761 ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc
    11821 tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg
    11881 gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    11941 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    12001 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    12061 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    12121 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    12181 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    12241 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    12301 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    12361 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    12421 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    12481 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    12541 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    12601 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    12661 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    12721 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    12781 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    12841 ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg
    12901 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
    12961 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
    13021 ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac
    13081 accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga
    13141 tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa
    13201 aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg
    13261 cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt
    13321 acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg
    13381 cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg
    13441 atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat
    13501 atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac
    13561 ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc
    13621 tgaacataaa acactatcaa taagttggag tcattaccca attatgatag aatttacaag
    13681 ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc
    13741 attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata
    13801 cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg
    13861 caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct
    13921 tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct
    13981 atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg gcacccgcga
    14041 ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg
    14101 ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca ctgcccggca
    14161 cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc
    14221 tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg
    14281 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
    14341 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
    14401 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
    14461 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
    14521 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
    14581 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
    14641 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
    14701 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
    14761 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
    14821 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
    14881 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    14941 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt
    15001 gccttgctcg tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg
    15061 catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc
    15121 atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct
    15181 cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt
    15241 cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg
    15301 ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg
    15361 ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc
    15421 gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt
    15481 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
    15541 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
    15601 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
    15661 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
    15721 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc cacgcttccc
    15781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    15841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    15901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    15961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    16021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    16081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    16141 cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg
    16201 aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt
    16261 gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt
    16321 caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga
    16381 caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta
    16441 tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg
    16501 catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
    16561 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
    16621 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
    16681 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
    16741 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
    16801 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
    16861 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
    16921 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
    16981 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcg
//
