LOCUS       sequence-31354-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-HSP90 (3H80)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(812..830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      826..873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcctctgaat attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca
       61 cagcagcggc agagaaaact tgtatttcca gggcatgacg gagacgttcg cgttccaggc
      121 ggagatcaat cagttgatgt cgctgatcat caacaccttc tactcgaaca aggagatctt
      181 cctgcgcgag ctgatcagca atgcgtcgga tgcgtgcgac aagatccgct accagagcct
      241 gacggacccg tcggtgctgg gcgagtcgcc tcgcctgtgc atccgcgtgg tgccggacaa
      301 ggagaacaag acgctgacgg tggaggataa cggcatcggt atgaccaagg cggacctcgt
      361 gaacaacctg ggcacgatcg cgcgctccgg cacgaaggcg ttcatggaag cgctggaggc
      421 cggcggcgac atgagcatga tcggccagtt cggtgtcggc ttctactcgg cgtaccttgt
      481 ggcggaccgc gtgacggtga cgtcgaagaa caactccgac gagtcgtacg tatgggagtc
      541 gtccgcgggc ggcacgttca cgatcacgag cacgccggag tcggacatga agcgcggcac
      601 gcgcatcacg ctgcacctca aggaggacca gatggagtac ctggagccgc gccgcctgaa
      661 ggagctgatc aagaagcact ccgagttcat cggctacgac atcgagctga tggtggagaa
      721 gacgacggag aagtgatgac gaagcttgcg gccgcactcg aggatccggc tgctaacaaa
      781 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      841 ggggcctcta aacgggtctt gaggggtttt ttgctgaagg aggaactata tccggatatc
      901 ccgcaaga
//
