LOCUS       sequence-313541-       11215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Daniloski Z, Jordan TX, Wessels HH, Hoagland DA, Kasela S, Legut M, 
            Maniatis S, Mimitou EP, Lu L, Geller E, Danziger O, Rosenberg BR, 
            Phatnani H, Smibert P, Lappalainen T, tenOever BR, Sanjana NE
  TITLE     Identification of Required Host Factors for SARS-CoV-2 Infection in 
            Human Cells.
  JOURNAL   Cell. 2020 Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030.
  PUBMED    33147445
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030."
COMMENT     Sequence Label: pLenti-hACE2-hygro
FEATURES             Location/Qualifiers
     source          1..11215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3736..3792
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3813..4401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3866..3886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4284..4295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4473..4706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4784..4905
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4821..4840)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4875..4894
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4945..5080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5100..5119)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5101..5119)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5129..5146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5129..5145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5138..5160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5287..5742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5374..5393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5584..5605
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5768..5872
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5873..5941,5942..6733)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6091..6110)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6904..7492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7393..7412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7646..7663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7780..7801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7816..7821,7822..7839,7840..7846)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7859..7881
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7878..7894
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7878..7894
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7913..7933
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7915..7933
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7961..8187
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8188..8368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8415..8540
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9033..9266
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9451..9495
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9726..9936
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9748..9944
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9795..9930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9966..10985
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    11056..11173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggccac catgtcaagc tcttcctggc tccttctcag ccttgttgct
     1321 gtaactgctg ctcagtccac cattgaggaa caggccaaga catttttgga caagtttaac
     1381 cacgaagccg aagacctgtt ctatcaaagt tcacttgctt cttggaatta taacaccaat
     1441 attactgaag agaatgtcca aaacatgaat aatgctgggg acaaatggtc tgccttttta
     1501 aaggaacagt ccacacttgc ccaaatgtat ccactacaag aaattcagaa tctcacagtc
     1561 aagcttcagc tgcaggctct tcagcaaaat gggtcttcag tgctctcaga agacaagagc
     1621 aaacggttga acacaattct aaatacaatg agcaccatct acagtactgg aaaagtttgt
     1681 aacccagata atccacaaga atgcttatta cttgaaccag gtttgaatga aataatggca
     1741 aacagtttag actacaatga gaggctctgg gcttgggaaa gctggagatc tgaggtcggc
     1801 aagcagctga ggccattata tgaagagtat gtggtcttga aaaatgagat ggcaagagca
     1861 aatcattatg aggactatgg ggattattgg agaggagact atgaagtaaa tggggtagat
     1921 ggctatgact acagccgcgg ccagttgatt gaagatgtgg aacatacctt tgaagagatt
     1981 aaaccattat atgaacatct tcatgcctat gtgagggcaa agttgatgaa tgcctatcct
     2041 tcctatatca gtccaattgg atgcctccct gctcatttgc ttggtgatat gtggggtaga
     2101 ttttggacaa atctgtactc tttgacagtt ccctttggac agaaaccaaa catagatgtt
     2161 actgatgcaa tggtggacca ggcctgggat gcacagagaa tattcaagga ggccgagaag
     2221 ttctttgtat ctgttggtct tcctaatatg actcaaggat tctgggaaaa ttccatgcta
     2281 acggacccag gaaatgttca gaaagcagtc tgccatccca cagcttggga cctggggaag
     2341 ggcgacttca ggatccttat gtgcacaaag gtgacaatgg acgacttcct gacagctcat
     2401 catgagatgg ggcatatcca gtatgatatg gcatatgctg cacaaccttt tctgctaaga
     2461 aatggagcta atgaaggatt ccatgaagct gttggggaaa tcatgtcact ttctgcagcc
     2521 acacctaagc atttaaaatc cattggtctt ctgtcacccg attttcaaga agacaatgaa
     2581 acagaaataa acttcctgct caaacaagca ctcacgattg ttgggactct gccatttact
     2641 tacatgttag agaagtggag gtggatggtc tttaaagggg aaattcccaa agaccagtgg
     2701 atgaaaaagt ggtgggagat gaagcgagag atagttgggg tggtggaacc tgtgccccat
     2761 gatgaaacat actgtgaccc cgcatctctg ttccatgttt ctaatgatta ctcattcatt
     2821 cgatattaca caaggaccct ttaccaattc cagtttcaag aagcactttg tcaagcagct
     2881 aaacatgaag gccctctgca caaatgtgac atctcaaact ctacagaagc tggacagaaa
     2941 ctgttcaata tgctgaggct tggaaaatca gaaccctgga ccctagcatt ggaaaatgtt
     3001 gtaggagcaa agaacatgaa tgtaaggcca ctgctcaact actttgagcc cttatttacc
     3061 tggctgaaag accagaacaa gaattctttt gtgggatgga gtaccgactg gagtccatat
     3121 gcagaccaaa gcatcaaagt gaggataagc ctaaaatcag ctcttggaga taaagcatat
     3181 gaatggaacg acaatgaaat gtacctgttc cgatcatctg ttgcatatgc tatgaggcag
     3241 tactttttaa aagtaaaaaa tcagatgatt ctttttgggg aggaggatgt gcgagtggct
     3301 aatttgaaac caagaatctc ctttaatttc tttgtcactg cacctaaaaa tgtgtctgat
     3361 atcattccta gaactgaagt tgaaaaggcc atcaggatgt cccggagccg tatcaatgat
     3421 gctttccgtc tgaatgacaa cagcctagag tttctgggga tacagccaac acttggacct
     3481 cctaaccagc cccctgtttc catatggctg attgtttttg gagttgtgat gggagtgata
     3541 gtggttggca ttgtcatcct gatcttcact gggatcagag atcggaagaa gaaaaataaa
     3601 gcaagaagtg gagaaaatcc ttatgcctcc atcgatatta gcaaaggaga aaataatcca
     3661 ggattccaaa acactgatga tgttcagacc tcctttggta ccgagacctc ccaggtggcg
     3721 cccgctggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     3781 aatcctggac cgtaaccacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     3841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4201 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4321 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4381 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     4441 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4501 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4681 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     4741 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     4801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     4921 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4981 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5041 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5101 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5161 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5221 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5281 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5461 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5521 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5581 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5641 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5701 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     5761 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5821 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5881 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5941 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6001 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6061 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7861 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7921 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     7981 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8041 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     8101 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8161 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8341 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8401 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8461 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8521 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8581 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8641 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8701 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8761 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8821 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8881 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8941 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9001 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9061 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9121 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9181 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9241 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9301 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9361 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9421 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9481 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9541 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9601 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9661 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9721 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
     9781 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9841 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9901 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
     9961 ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg atcgaaaagt
    10021 tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg gctttcagct
    10081 tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat ggtttctaca
    10141 aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc gaagtgcttg
    10201 acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
    10261 ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc gccgaggcca
    10321 tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca ttcggacctc
    10381 aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct gatccccatg
    10441 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc caggctctcg
    10501 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgccgatt
    10561 tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt gactggagcg
    10621 aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccctggt
    10681 tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag cttgcaggat
    10741 ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat cagagcttgg
    10801 ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca atcgtccggt
    10861 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc gtctggaccg
    10921 atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact cgtcctaggg
    10981 caaaggatct gcagtaatga ggcgcgccgt taacgaattc tagatcttga gacaaatggc
    11041 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    11101 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11161 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccgg
//
