LOCUS       sequence-313549-        6234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pDTA-TK
FEATURES             Location/Qualifiers
     source          1..6234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             893..1465
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          1542..1607
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1668..1802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1705..1724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1759..1778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1822..1838
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2113..2175
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           2197..2236
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             2254..3384
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(3382..3401)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3426..3474
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      5046..5055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5099..5203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5204..5272,5273..6064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5422..5441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttct ggccttttgc acggaccgaa caactccgcc gcgcgcgctc
      661 cgtgtaggcc ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg
      721 acacaagggc gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag
      781 agcgcgaccg ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc
      841 gtaacctctg ccgttcagga tctgcgacct gcaggtcctc gccatggacc ctgatgatgt
      901 tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg ggactaaacc
      961 tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa
     1021 ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata
     1081 ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta
     1141 tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga
     1201 gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa
     1261 aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc
     1321 tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat
     1381 taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc
     1441 ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga
     1501 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1561 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1621 gaactgatga atgggagcag tggtggaatg cagatccact aggatctaac ttgtttattg
     1681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1801 tcgtagttct agagcggacc ggtaaaacga cggccagtgt taactctagg aattctctag
     1861 ggtacctcta ggagatcgat cctctagagt cgagcagtgt ggttttcaag aggaagcaaa
     1921 aagcctctcc acccaggcct ggaatgtttc cacccaatgt cgagcagtgt ggttttgcaa
     1981 gaggaagcaa aaagcctctc cacccaggcc tggaatgttt ccacccaatg tcgagcaaac
     2041 cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg
     2101 tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg
     2161 cagcgacccg cttaacagcg tcaacagcgt gccgcagatc ttggtggcgt gaaactcccg
     2221 cacctcttcg gccagcgcct tgtagaagcg cgtatggctt cgtaccccgg ccatcagcac
     2281 gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata gcaaccgacg tacggcgttg
     2341 cgccctcgcc ggcagcaaga agccacggaa gtccgcccgg agcagaaaat gcccacgcta
     2401 ctgcgggttt atatagacgg tccccacggg atggggaaaa ccaccaccac gcaactgctg
     2461 gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcgg
     2521 gtgctggggg cttccgagac aatcgcgaac atctacacca cacaacaccg ccttgaccag
     2581 ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc
     2641 atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc
     2701 tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc
     2761 ctgtgctacc cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg
     2821 ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg
     2881 gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg
     2941 gctatgctgg ccgcgattcg ccgcgtttac gggctgcttg ccaatacggt gcggtatctg
     3001 cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt cggggacggc cgtgccgccc
     3061 cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt
     3121 accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtacaa cgtgtttgcc
     3181 tgggccttgg acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac
     3241 gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatgatccag
     3301 acccacgtca ccaccccagg ctccataccg acgatctgcg acctggcgcg cacgtttgcc
     3361 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     3421 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     3481 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     3541 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     3601 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cacgggcccc gtgggttagg
     3661 gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc
     3721 gtggggtcag tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     3781 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     3841 gacccccaaa aaccaccgcg cggatttctg gcgccgccgg acgaactaaa cctgactacg
     3901 gcatctctgc cccttcttcg ctggtacgag gagcgctttt gttttgtatt ggtcaccacg
     3961 gccgagtttc cgcgggaccc cggccagatc aagctagagt ctgaaagaaa aaacacaatc
     4021 tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
     4081 gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
     4141 ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
     4201 cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
     4261 ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
     4321 cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
     4381 ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
     4441 ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
     4501 ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctggc
     4561 gcgtttcttc cagcagccag ccctgcgtga tgtcaccatt gaagagttca acgctgttgc
     4621 gcatgaactg gacccgatcg tggcaaaacg cgtgcgtcat atactgactg aaaacgcccg
     4681 caccgttgaa gctgccagcg cgctggagca aggcgacctg aaacgtatgg gcgagttgat
     4741 ggcggagtct catgcctcta tgcgcgatga tttcgaaatc accgtgccgc aaattgacac
     4801 tctggtagaa atcgtcaaag ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg
     4861 cggatttggc ggctgtatcg tcgcgctgat cccggaagag ctggtgcctg ccgtacagca
     4921 agctgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt acgtttgtaa
     4981 accatcacaa ggagcaggac agtgcttatc ggatcttcgc taccttagga ccgttatagt
     5041 tagcggccgc catggtcata gctgtttgac gtcaggtggc acttttcggg gaaatgtgcg
     5101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcacgagaca
     5161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6121 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
