LOCUS       sequence-313559-       13467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, 
            Whelan SP, Sabatini B, Cepko CL
  TITLE     Anterograde or retrograde transsynaptic labeling of CNS neurons with
            vesicular stomatitis virus vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug
            8.
  PUBMED    21825165
REFERENCE   2  (bases 1 to 13467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-09-13"; volume: "108(37)"; pages: 
            "15414-9. Epub 2011 Aug 8"
COMMENT     Sequence Label: pVSV eGFP dG
FEATURES             Location/Qualifiers
     source          1..13467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(92..94,95..97,98..811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(92..94,95..97,98..808)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(137..158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(398..417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     745..766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10554..10572)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10568..10615
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(10683..10701)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10684..10701)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10991..11010)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11220..11239)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11339..11361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11399..11417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11485..11589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11590..11658,11659..12450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11808..11827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12621..13209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13110..13129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13363..13380
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactatagga cgaagacaaa caaaccatta ttatcattaa aaggctcagg
       61 agaaacttta acagtaatca gaattctcga gatggtgagc aagggcgagg agctgttcac
      121 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      181 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      241 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      301 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      361 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      421 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      481 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      541 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      601 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      661 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
      721 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      781 cactctcggc atggacgagc tgtacaagtg atggccatat gaaaaaaact aacagtaatc
      841 aaaatgtctg ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct
      901 gcaaatgagg atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct
      961 ctttacatca atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc
     1021 aaatccggaa atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac
     1081 atccggggta agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg
     1141 gatacaatcg gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat
     1201 ggagtatcgg atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt
     1261 ggcttataca gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg
     1321 ctgacaaatc aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt
     1381 gacatttttg atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac
     1441 atgttcttcc acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt
     1501 tccagattca aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga
     1561 atgtctacag aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc
     1621 caaatgatgc ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc
     1681 gactttggat tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc
     1741 tgggggcaat tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct
     1801 gatgacattg agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga
     1861 tcctctgccg acttggcaca acagttttgt gttggagata acaaatacac tccagatgat
     1921 agtaccggag gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc
     1981 ggatggtttg aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga
     2041 gcagtcatgt cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa
     2101 tttgacaaat gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa
     2161 aaactaacag atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctactct
     2221 cgtctagatc aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc
     2281 aattatgagt tgttccaaga ggacggagtg gaagagcata ctaggccctc ttattttcag
     2341 gcagcagatg attctgacac agaatctgaa ccagaaattg aagacaatca aggcttgtat
     2401 gtaccagatc cggaagctga gcaagttgaa ggctttatac aggggccttt agatgactat
     2461 gcagatgagg acgtggatgt tgtattcact tcggactgga aacagcctga gcttgaatcc
     2521 gacgagcatg gaaagacctt acggttgaca ttgccagagg gtttaagtgg agagcagaaa
     2581 tcccagtggc ttttgacgat taaagcagtc gttcaaagtg ccaaacactg gaatctggca
     2641 gagtgcacat ttgaagcatc gggagaaggg gtcatcataa aaaagcgcca gataactccg
     2701 gatgtatata aggtcactcc agtgatgaac acacatccgt accaatcaga agccgtatca
     2761 gatgtttggt ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag
     2821 cctctcacca tatccttgga tgaattgttc tcatctagag gagaattcat ctctgtcgga
     2881 ggtaacggac gaatgtctca taaagaggcc atcctgctcg gtctgaggta caaaaagttg
     2941 tacaatcagg cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac
     3001 aatctaagtg ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga
     3061 aggggaaagg taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca
     3121 ctagcatgga gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga
     3181 tggacaccta tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga
     3241 cggttagatc taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt
     3301 gggatcacat gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt
     3361 tgggttcttc taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt
     3421 atcacgctca ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca
     3481 tgctcaatgt accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga
     3541 ttgagctcac aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg
     3601 atcatttcaa ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga
     3661 ttgtcgagaa aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag
     3721 ctagtctagc ttccagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc
     3781 ctttcgaaca actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga
     3841 tctgtttcct tgacacgcgt tacgatgcgg ccgccctgca cacagattct tcatgtttga
     3901 accaaatcaa cttgtgatat catgctcaaa gaggccttaa ttatatttta atttttaatt
     3961 tttatgaaaa aaactaacag caatcatgga agtccacgat tttgagaccg acgagttcaa
     4021 tgatttcaat gaagatgact atgccacaag agaattcctg aatcccgatg agcgcatgac
     4081 gtacttgaat catgctgatt acaatttgaa ttctcctcta attagtgatg atattgacaa
     4141 tttgatcagg aaattcaatt ctcttccgat tccctcgatg tgggatagta agaactggga
     4201 tggagttctt gagatgttaa catcatgtca agccaatccc atctcaacat ctcagatgca
     4261 taaatggatg ggaagttggt taatgtctga taatcatgat gccagtcaag ggtatagttt
     4321 tttacatgaa gtggacaaag aggcagaaat aacatttgac gtggtggaga ccttcatccg
     4381 cggctggggc aacaaaccaa ttgaatacat caaaaaggaa agatggactg actcattcaa
     4441 aattctcgct tatttgtgtc aaaagttttt ggacttacac aagttgacat taatcttaaa
     4501 tgctgtctct gaggtggaat tgctcaactt ggcgaggact ttcaaaggca aagtcagaag
     4561 aagttctcat ggaacgaaca tatgcaggat tagggttccc agcttgggtc ctacttttat
     4621 ttcagaagga tgggcttact tcaagaaact tgatattcta atggaccgaa actttctgtt
     4681 aatggtcaaa gatgtgatta tagggaggat gcaaacggtg ctatccatgg tatgtagaat
     4741 agacaacctg ttctcagagc aagacatctt ctcccttcta aatatctaca gaattggaga
     4801 taaaattgtg gagaggcagg gaaatttttc ttatgacttg attaaaatgg tggaaccgat
     4861 atgcaacttg aagctgatga aattagcaag agaatcaagg cctttagtcc cacaattccc
     4921 tcattttgaa aatcatatca agacttctgt tgatgaaggg gcaaaaattg accgaggtat
     4981 aagattcctc catgatcaga taatgagtgt gaaaacagtg gatctcacac tggtgattta
     5041 tggatcgttc agacattggg gtcatccttt tatagattat tacactggac tagaaaaatt
     5101 acattcccaa gtaaccatga agaaagatat tgatgtgtca tatgcaaaag cacttgcaag
     5161 tgatttagct cggattgttc tatttcaaca gttcaatgat cataaaaagt ggttcgtgaa
     5221 tggagacttg ctccctcatg atcatccctt taaaagtcat gttaaagaaa atacatggcc
     5281 cacagctgct caagttcaag attttggaga taaatggcat gaacttccgc tgattaaatg
     5341 ttttgaaata cccgacttac tagacccatc gataatatac tctgacaaaa gtcattcaat
     5401 gaataggtca gaggtgttga aacatgtccg aatgaatccg aacactccta tccctagtaa
     5461 aaaggtgttg cagactatgt tggacacaaa ggctaccaat tggaaagaat ttcttaaaga
     5521 gattgatgag aagggcttag atgatgatga tctaattatt ggtcttaaag gaaaggagag
     5581 ggaactgaag ttggcaggta gatttttctc cctaatgtct tggaaattgc gagaatactt
     5641 tgtaattacc gaatatttga taaagactca tttcgtccct atgtttaaag gcctgacaat
     5701 ggcggacgat ctaactgcag tcattaaaaa gatgttagat tcctcatccg gccaaggatt
     5761 gaagtcatat gaggcaattt gcatagccaa tcacattgat tacgaaaaat ggaataacca
     5821 ccaaaggaag ttatcaaacg gcccagtgtt ccgagttatg ggccagttct taggttatcc
     5881 atccttaatc gagagaactc atgaattttt tgagaaaagt cttatatact acaatggaag
     5941 accagacttg atgcgtgttc acaacaacac actgatcaat tcaacctccc aacgagtttg
     6001 ttggcaagga caagagggtg gactggaagg tctacggcaa aaaggatgga gtatcctcaa
     6061 tctactggtt attcaaagag aggctaaaat cagaaacact gctgtcaaag tcttggcaca
     6121 aggtgataat caagttattt gcacacagta taaaacgaag aaatcgagaa acgttgtaga
     6181 attacagggt gctctcaatc aaatggtttc taataatgag aaaattatga ctgcaatcaa
     6241 aatagggaca gggaagttag gacttttgat aaatgacgat gagactatgc aatctgcaga
     6301 ttacttgaat tatggaaaaa taccgatttt ccgtggagtg attagagggt tagagaccaa
     6361 gagatggtca cgagtgactt gtgtcaccaa tgaccaaata cccacttgtg ctaatataat
     6421 gagctcagtt tccacaaatg ctctcaccgt agctcatttt gctgagaacc caatcaatgc
     6481 catgatacag tacaattatt ttgggacatt tgctagactc ttgttgatga tgcatgatcc
     6541 tgctcttcgt caatcattgt atgaagttca agataagata ccgggcttgc acagttctac
     6601 tttcaaatac gccatgttgt atttggaccc ttccattgga ggagtgtcgg gcatgtcttt
     6661 gtccaggttt ttgattagag ccttcccaga tcccgtaaca gaaagtctct cattctggag
     6721 attcatccat gtacatgctc gaagtgagca tctgaaggag atgagtgcag tatttggaaa
     6781 ccccgagata gccaagtttc gaataactca catagacaag ctagtagaag atccaacctc
     6841 tctgaacatc gctatgggaa tgagtccagc gaacttgtta aagactgagg ttaaaaaatg
     6901 cttaatcgaa tcaagacaaa ccatcaggaa ccaggtgatt aaggatgcaa ccatatattt
     6961 gtatcatgaa gaggatcggc tcagaagttt cttatggtca ataaatcctc tgttccctag
     7021 atttttaagt gaattcaaat caggcacttt tttgggagtc gcagacgggc tcatcagtct
     7081 atttcaaaat tctcgtacta ttcggaactc ctttaagaaa aagtatcata gggaattgga
     7141 tgatttgatt gtgaggagtg aggtatcctc tttgacacat ttagggaaac ttcatttgag
     7201 aaggggatca tgtaaaatgt ggacatgttc agctactcat gctgacacat taagatacaa
     7261 atcctggggc cgtacagtta ttgggacaac tgtaccccat ccattagaaa tgttgggtcc
     7321 acaacatcga aaagagactc cttgtgcacc atgtaacaca tcagggttca attatgtttc
     7381 tgtgcattgt ccagacggga tccatgacgt ctttagttca cggggaccat tgcctgctta
     7441 tctagggtct aaaacatctg aatctacatc tattttgcag ccttgggaaa gggaaagcaa
     7501 agtcccactg attaaaagag ctacacgtct tagagatgct atctcttggt ttgttgaacc
     7561 cgactctaaa ctagcaatga ctatactttc taacatccac tctttaacag gcgaagaatg
     7621 gaccaaaagg cagcatgggt tcaaaagaac agggtctgcc cttcataggt tttcgacatc
     7681 tcggatgagc catggtgggt tcgcatctca gagcactgca gcattgacca ggttgatggc
     7741 aactacagac accatgaggg atctgggaga tcagaatttc gactttttat tccaagcaac
     7801 gttgctctat gctcaaatta ccaccactgt tgcaagagac ggatggatca ccagttgtac
     7861 agatcattat catattgcct gtaagtcctg tttgagaccc atagaagaga tcaccctgga
     7921 ctcaagtatg gactacacgc ccccagatgt atcccatgtg ctgaagacat ggaggaatgg
     7981 ggaaggttcg tggggacaag agataaaaca gatctatcct ttagaaggga attggaagaa
     8041 tttagcacct gctgagcaat cctatcaagt cggcagatgt ataggttttc tatatggaga
     8101 cttggcgtat agaaaatcta ctcatgccga ggacagttct ctatttcctc tatctataca
     8161 aggtcgtatt agaggtcgag gtttcttaaa agggttgcta gacggattaa tgagagcaag
     8221 ttgctgccaa gtaatacacc ggagaagtct ggctcatttg aagaggccgg ccaacgcagt
     8281 gtacggaggt ttgatttact tgattgataa attgagtgta tcacctccat tcctttctct
     8341 tactagatca ggacctatta gagacgaatt agaaacgatt ccccacaaga tcccaacctc
     8401 ctatccgaca agcaaccgtg atatgggggt gattgtcaga aattacttca aataccaatg
     8461 ccgtctaatt gaaaagggaa aatacagatc acattattca caattatggt tattctcaga
     8521 tgtcttatcc atagacttca ttggaccatt ctctatttcc accaccctct tgcaaatcct
     8581 atacaagcca tttttatctg ggaaagataa gaatgagttg agagagctgg caaatctttc
     8641 ttcattgcta agatcaggag aggggtggga agacatacat gtgaaattct tcaccaagga
     8701 catattattg tgtccagagg aaatcagaca tgcttgcaag ttcgggattg ctaaggataa
     8761 taataaagac atgagctatc ccccttgggg aagggaatcc agagggacaa ttacaacaat
     8821 ccctgtttat tatacgacca ccccttaccc aaagatgcta gagatgcctc caagaatcca
     8881 aaatcccctg ctgtccggaa tcaggttggg ccaattacca actggcgctc attataaaat
     8941 tcggagtata ttacatggaa tgggaatcca ttacagggac ttcttgagtt gtggagacgg
     9001 ctccggaggg atgactgctg cattactacg agaaaatgtg catagcagag gaatattcaa
     9061 tagtctgtta gaattatcag ggtcagtcat gcgaggcgcc tctcctgagc cccccagtgc
     9121 cctagaaact ttaggaggag ataaatcgag atgtgtaaat ggtgaaacat gttgggaata
     9181 tccatctgac ttatgtgacc caaggacttg ggactatttc ctccgactca aagcaggctt
     9241 ggggcttcaa attgatttaa ttgtaatgga tatggaagtt cgggattctt ctactagcct
     9301 gaaaattgag acgaatgtta gaaattatgt gcaccggatt ttggatgagc aaggagtttt
     9361 aatctacaag acttatggaa catatatttg tgagagcgaa aagaatgcag taacaatcct
     9421 tggtcccatg ttcaagacgg tcgacttagt tcaaacagaa tttagtagtt ctcaaacgtc
     9481 tgaagtatat atggtatgta aaggtttgaa gaaattaatc gatgaaccca atcccgattg
     9541 gtcttccatc aatgaatcct ggaaaaacct gtacgcattc cagtcatcag aacaggaatt
     9601 tgccagagca aagaaggtta gtacatactt taccttgaca ggtattccct cccaattcat
     9661 tcctgatcct tttgtaaaca ttgagactat gctacaaata ttcggagtac ccacgggtgt
     9721 gtctcatgcg gctgccttaa aatcatctga tagacctgca gatttattga ccattagcct
     9781 tttttatatg gcgattatat cgtattataa catcaatcat atcagagtag gaccgatacc
     9841 tccgaacccc ccatcagatg gaattgcaca aaatgtgggg atcgctataa ctggtataag
     9901 cttttggctg agtttgatgg agaaagacat tccactatat caacagtgtt tggcagttat
     9961 ccagcaatca tttccgatta ggtgggaggc tatttcagta aaaggaggat acaagcagaa
    10021 gtggagtact agaggtgatg ggctcccaaa agatacccga atttcagact ccttggcccc
    10081 aatcgggaac tggatcagat ctttggaatt ggtccgaaac caagttcgtc taaatccatt
    10141 caataagatc ttgttcaatc agctatgtcg tacagtggat aatcatttga agtggtcaaa
    10201 tttgcgaaaa aacacaggaa tgattgaatg gatcaatggg cgaatttcaa aagaagaccg
    10261 gtctatactg atgttgaaga gtgacctaca tgaggaaaac tcttggagag attaaaaaat
    10321 caggaggaga ctccaaactt taagtatgaa aaaaactttg atccttaaga ccctcttgtg
    10381 gtttttattt ttttatctgg ttttgtggtc ttcgtgggtc ggcatggcat ctccacctcc
    10441 tcgcggtccg acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag
    10501 ctcggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
    10561 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
    10621 aggaggaact atatccggat cgagatcctc tagagtcgac ctgcaggcat gcaagcttgt
    10681 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
    10741 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
    10801 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
    10861 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
    10921 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
    10981 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
    11041 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
    11101 ggcgccatct ccttgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
    11161 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
    11221 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    11281 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    11341 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    11401 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    11461 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    11521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    11581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    11641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    11701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    11761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    11821 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    11881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    11941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    12001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    12061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    12121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    12181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    12241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    12301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    12361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    12421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    12481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    12541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    12601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    12661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    12721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    12781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    12841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    12901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    12961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    13021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    13081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    13141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    13201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    13261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    13321 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    13381 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    13441 tgcaggggga tctcgatccc gcgaaat
//
