LOCUS       sequence-313571-        1727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for endogenous tagging of human FUS at the N-terminus 
            with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1727)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUS_Halo_N_allele_400
FEATURES             Location/Qualifiers
     source          1..1727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             395..1285
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ccagccgccg ggaccgcccc ctcgccccgc ccccagcggg cactcaggcc gtaccactgt
       61 gccttcatgg gggtggagat agatcgtggg ctagtcctgc cgaggagaga ggggttcttc
      121 ctcaaaaaat atgattatgt atagtattcg catgattcta gttaacttgt ttcccttctg
      181 cctgctcgga ccctctacct gccctacgaa gggggcggag tgcgttcctg cctccccctg
      241 ctcttccgcg tttggtgcgc gcctgcgcgg tgcgtaggcg gcggagcgta cttaagcttc
      301 gacgcaggag gcggggctgc tcagtcctcc aggcgtcggt actcagcggt gttggaactt
      361 cgttgcttgc ttgcctgtgc gcgcgtgcgc ggacatggcc gagatcggca ccggctttcc
      421 cttcgatcct cactacgtgg aagtgctggg cgagagaatg cactatgtgg acgtgggccc
      481 cagagatgga acccctgtgc tgtttctgca cggcaaccct accagcagct acgtgtggcg
      541 gaacatcatc cctcacgtgg cccctacaca cagatgtatc gcccctgacc tgatcggcat
      601 gggcaagagc gataagcccg acctgggcta cttcttcgac gaccacgtgc ggttcatgga
      661 cgcctttatt gaggccctcg gcctggaaga ggtggtgctg gttattcacg attggggcag
      721 cgccctggga ttccactggg ccaagagaaa ccccgagaga gtgaagggaa tcgccttcat
      781 ggaattcatc aggcccattc ctacctggga cgagtggcct gagttcgcca gagagacatt
      841 ccaggccttc agaaccaccg acgtgggcag aaagctgatc atcgaccaga acgtgttcat
      901 cgagggcacc ctgcctatgg gagtcgtcag accactgacc gaggtggaaa tggaccacta
      961 cagagagccc tttctgaacc ccgtggaccg ggaacctctt tggagattcc ccaacgagct
     1021 gcccattgcc ggcgagcctg ctaatattgt ggccctggtg gaagagtaca tggactggct
     1081 gcatcagagc cccgtgccta agctgctgtt ttggggaaca cccggcgtgc tgattcctcc
     1141 tgctgaagct gccagactgg ccaagagcct gcctaattgc aaggccgtgg atatcggccc
     1201 tggcctgaat ctgctgcaag aggacaaccc cgatctgatc ggctccgaga ttgccagatg
     1261 gctgagcacc ctggaaatca gcggaggagg aggttctgga ggcggtggaa gtggtggcgg
     1321 aggtagctca aacggtaggt aagggcgcga ggcgacggcg gcggcgcacc cggccgaggc
     1381 ctcccagctg ggcttttcgt tttcagtggg accggggcgg cgatcccgtg tgggattttt
     1441 tggcgcccct gtggcgggaa gccgcggaga agagtaactg gaggaggctg gtgtcgccat
     1501 tttgtttcgc tcctctggcc ctcgcgcgcg gggcgggaag tcttttcttt gcagtccgtt
     1561 tgcttggggt gggcgttggg agggacgctt cttaggggtt tgaagcgtca ggtgagggtg
     1621 gaaaacgccc attctccgtg gcctcgcctc ccccaactcc cggccccgcg ctcgagcccg
     1681 ctttgtcgca gtgctgcatc cgggcactcg cggcgcgcac gcgctct
//
