LOCUS       sequence-313576-        9979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutive expression of 
            V5-tagged OAS2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pLX302 OAS2-V5 puro
FEATURES             Location/Qualifiers
     source          1..9979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        96..399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..603
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     554..574
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    626..650
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2712..2736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2738..2779
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2821..3409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2874..2894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3292..3303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3481..3714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3792..3913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3829..3848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3883..3902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3953..4088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4108..4127)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4109..4127)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4137..4154)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4137..4153)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4146..4168)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4295..4750
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4382..4401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4592..4613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4776..4880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4881..4949,4950..5741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5099..5118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5912..6500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6401..6420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6654..6671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6788..6809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6824..6829,6830..6847,6848..6854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6862..6878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6867..6889
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6886..6902
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6886..6902
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6921..6941
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6923..6941
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6969..7195
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7196..7376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7423..7548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8041..8274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8459..8503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8678..9178
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9097..9115
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9200..9799
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9200..9219)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9696..9716
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9841..9958
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta atcaattacg
       61 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      121 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      181 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      241 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      301 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      361 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      421 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      481 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      541 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      601 gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca tgggaaatgg
      661 ggagtcccag ctgtcctcgg tgcctgctca gaagctgggt tggtttatcc aggaatacct
      721 gaagccctac gaagaatgtc agacactgat cgacgagatg gtgaacacca tctgtgacgt
      781 cctgcaggaa cccgaacagt tccccctggt gcagggagtg gccataggtg gctcctatgg
      841 acggaaaaca gtcttaagag gcaactccga tggtaccctt gtcctcttct tcagtgactt
      901 aaaacaattc caggatcaga agagaagcca acgtgacatc ctcgataaaa ctggggataa
      961 gctgaagttc tgtctgttca cgaagtggtt gaaaaacaat ttcgagatcc agaagtccct
     1021 tgatgggttc accatccagg tgttcacaaa aaatcagaga atctctttcg aggtgctggc
     1081 cgccttcaac gctctgagct taaatgataa tcccagcccc tggatctatc gagagctcaa
     1141 aagatccttg gataagacaa atgccagtcc tggtgagttt gcagtctgct tcactgaact
     1201 ccagcagaag ttttttgaca accgtcctgg aaaactaaag gatttgatcc tcttgataaa
     1261 gcactggcat caacagtgcc agaaaaaaat caaggattta ccctcgctgt ctccgtatgc
     1321 cctggagctg cttacggtgt atgcctggga acaggggtgc agaaaagaca actttgacat
     1381 tgctgaaggc gtcagaaccg ttctggagct gatcaaatgc caggagaagc tgtgtatcta
     1441 ttggatggtc aactacaact ttgaagatga gaccatcagg aacatcctgc tgcaccagct
     1501 ccaatcagcg aggccagtaa tcttggatcc agttgaccca accaataatg tgagtggaga
     1561 taaaatatgc tggcaatggc tgaaaaaaga agctcaaacc tggttgactt ctcccaacct
     1621 ggataatgag ttacctgcac catcttggaa tgttctgcct gcaccactct tcacgacccc
     1681 aggccacctt ctggataagt tcatcaagga gtttctccag cccaacaaat gcttcctaga
     1741 gcagattgac agtgctgtta acatcatccg tacattcctt aaagaaaact gcttccgaca
     1801 atcaacagcc aagatccaga ttgtccgggg aggatcaacc gccaaaggca cagctctgaa
     1861 gactggctct gatgccgatc tcgtcgtgtt ccataactca cttaaaagct acacctccca
     1921 aaaaaacgag cggcacaaaa tcgtcaagga aatccatgaa cagctgaaag ccttttggag
     1981 ggagaaggag gaggagcttg aagtcagctt tgagcctccc aagtggaagg ctcccagggt
     2041 gctgagcttc tctctgaaat ccaaagtcct caacgaaagt gtcagctttg atgtgcttcc
     2101 tgcctttaat gcactgggtc agctgagttc tggctccaca cccagccccg aggtttatgc
     2161 agggctcatt gatctgtata aatcctcgga cctcccggga ggagagtttt ctacctgttt
     2221 cacagtcctg cagcgaaact tcattcgctc ccggcccacc aaactaaagg atttaattcg
     2281 cctggtgaag cactggtaca aagagtgtga aaggaaactg aagccaaagg ggtctttgcc
     2341 cccaaagtat gccttggagc tgctcaccat ctatgcctgg gagcagggga gtggagtgcc
     2401 ggattttgac actgcagaag gtttccggac agtcctggag ctggtcacac aatatcagca
     2461 gctctgcatc ttctggaagg tcaattacaa ctttgaagat gagaccgtga ggaagtttct
     2521 actgagccag ttgcagaaaa ccaggcctgt gatcttggac ccagccgaac ccacaggtga
     2581 cgtgggtgga ggggaccgtt ggtgttggca tcttctggca aaagaagcaa aggaatggtt
     2641 atcctctccc tgcttcaagg atgggactgg aaacccaata ccaccttgga aagtgccggt
     2701 aaaagtcatc tacccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
     2761 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
     2821 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2881 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2941 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3001 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3061 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3121 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3181 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3241 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3301 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3361 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     3421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3481 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     3721 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3781 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3841 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3901 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     3961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4081 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4141 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4201 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4261 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4441 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4501 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4621 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4741 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5101 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5401 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5761 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5821 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5881 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5941 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6001 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6061 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6121 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6181 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6241 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6301 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6361 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6421 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6481 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6541 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6601 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6661 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6721 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6781 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6841 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6901 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6961 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7021 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7081 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7141 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7381 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7441 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7501 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7561 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7621 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7681 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7741 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7801 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7861 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7921 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7981 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8041 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8101 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8161 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8221 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8281 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8341 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8401 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8461 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8521 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8581 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8641 atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc
     8701 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     8761 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     8821 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     8881 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     8941 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
     9001 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     9061 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
     9121 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
     9181 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9241 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9301 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9361 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     9421 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9481 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9541 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9601 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9661 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9721 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9781 ccgcaagccc ggtgcctgag aattctagat cttgagacaa atggcagtat tcatccacaa
     9841 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     9901 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9961 ggtttattac agggacagc
//
