LOCUS       sequence-313577-        9463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutive expression of 
            V5-tagged OASL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pLX302 OASL-V5 puro
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        288..491
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     442..462
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    514..538
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2084..2108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2110..2151
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2193..2781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2246..2266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2664..2675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2853..3086
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3164..3285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3201..3220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3255..3274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3325..3460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3480..3499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3481..3499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3509..3526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3509..3525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3518..3540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3667..4122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3754..3773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3964..3985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4148..4252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4253..4321,4322..5113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4471..4490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5284..5872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5773..5792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6026..6043
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6160..6181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6196..6201,6202..6219,6220..6226)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6234..6250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6239..6261
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6258..6274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6258..6274
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6293..6313
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6295..6313
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6341..6567
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6568..6748
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6795..6920
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7413..7646
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7831..7875
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8050..8550
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8469..8487
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8572..9171
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8572..8591)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9068..9088
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9213..9330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9447..9463,1..287)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
       61 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      121 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      181 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      241 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      301 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      361 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      421 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      481 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agttggcacc
      541 atggcactga tgcaggaact gtatagcaca ccagcctcca ggctggactc cttcgtggct
      601 cagtggctgc agccccaccg ggagtggaag gaagaggtgc tagacgctgt gcggaccgtg
      661 gaggagtttc tgaggcagga gcatttccag gggaagcgtg ggctggacca ggatgtgcgg
      721 gtgctgaagg tagtcaaggt gggctccttc gggaatggca cggttctcag gagcaccaga
      781 gaggtggagc tggtggcgtt tctgagctgt ttccacagct tccaggaggc agccaagcat
      841 cacaaagatg ttctgaggct gatatggaaa accatgtggc aaagccagga cctgctggac
      901 ctcgggctcg aggacctgag gatggagcag agagtccccg atgctcttgt cttcaccatc
      961 cagaccaggg ggactgcgga gcccatcacg gtcaccattg tgcctgccta cagagccctg
     1021 gggccttctc ttcccaactc ccagccaccc cctgaggtct atgtgagcct gatcaaggcc
     1081 tgcggtggtc ctggaaattt ctgcccatcc ttcagcgagc tgcagagaaa tttcgtgaaa
     1141 catcggccaa ctaagctgaa gagcctcctg cgcctggtga aacactggta ccagcagtat
     1201 gtgaaagcca ggtcccccag agccaatctg ccccctctct atgctcttga acttctaacc
     1261 atctatgcct gggaaatggg tactgaagaa gacgagaatt tcatgttgga cgaaggcttc
     1321 accactgtga tggacctgct cctggagtat gaagtcatct gtatctactg gaccaagtac
     1381 tacacactcc acaatgcaat cattgaggat tgtgtcagaa aacagctcaa aaaagagagg
     1441 cccatcatcc tggatccggc cgaccccacc ctcaacgtgg cagaagggta cagatgggac
     1501 atcgttgctc agagggcctc ccagtgcctg aaacaggact gttgctatga caacagggag
     1561 aaccccatct ccagctggaa cgtgaagagg gcacgagaca tccacttgac agtggagcag
     1621 aggggttacc cagatttcaa cctcatcgtg aacccttatg agcccataag gaaggttaaa
     1681 gagaaaatcc ggaggaccag gggctactct ggcctgcagc gtctgtcctt ccaggttcct
     1741 ggcagtgaga ggcagcttct cagcagcagg tgctccttag ccaaatatgg gatcttctcc
     1801 cacactcaca tctatctgct ggagaccatc ccctccgaga tccaggtctt cgtgaagaat
     1861 cctgatggtg ggagctacgc ctatgccatc aaccccaaca gcttcatcct gggtctgaag
     1921 cagcagattg aagaccagca ggggcttcct aaaaagcagc agcagctgga attccaaggc
     1981 caagtcctgc aggactggtt gggtctgggg atctatggca tccaagacag tgacactctc
     2041 atcctctcga agaagaaagg agaggctctg tttccagcca gtttgccaac tttcttgtac
     2101 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     2161 gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     2221 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2281 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2341 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2401 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2461 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2521 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2581 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2641 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2701 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2761 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     2821 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     2881 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     2941 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3001 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3061 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     3121 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     3181 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3241 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     3301 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3361 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3421 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     3481 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3541 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3601 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3661 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3841 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3901 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3961 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4021 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4081 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4141 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4261 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4321 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4381 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4441 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4501 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4561 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4621 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4681 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4741 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4801 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4861 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4921 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4981 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5041 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5101 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5161 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5221 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5281 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5341 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5401 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5461 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5521 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5581 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5641 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5701 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5761 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5821 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5881 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5941 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6001 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6061 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6121 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6181 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6241 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     6301 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     6361 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     6421 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     6481 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     6541 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     6601 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6661 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6721 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6781 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6841 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     6901 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     6961 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7021 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7081 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7141 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7201 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7261 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7321 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7381 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7441 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7501 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7561 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7621 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7681 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7741 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7801 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7861 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     7921 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     7981 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8041 aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     8101 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     8161 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     8221 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     8281 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     8341 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     8401 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     8461 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     8521 gttgaccgaa tcaccgacct ctctccccag ggggtaccac cggagcttac catgaccgag
     8581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     8641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     8701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     8761 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     8821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     8881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     8941 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     9061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agaattctag
     9181 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     9241 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9301 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     9361 ctttggcgcc ggctcgaggg ggcccggggt tattaatagt aatcaattac ggggtcatta
     9421 gttcatagcc catatatgga gttccgcgtt acataactta cgg
//
