LOCUS       sequence-313584-       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, 
            mAID-tagged) driven by human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1aGFP2AKAT7-linker-mAID-W
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1194..1203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1200..1202,1203..1205,1206..1916)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1200..1202,1203..1205,1206..1916)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1245..1266)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1506..1525)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1853..1874
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1935..1988
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3840..4043
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     primer_bind     complement(4118..4138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4536..4547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4999..5016)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5005..5229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5255..5275)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5255..5273)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5294..5310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5294..5310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5307..5329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5318..5334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5342..5348,5349..5366,5367..5372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5387..5408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5525..5542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5696..6284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5776..5795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6455..7246,7247..7315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7078..7097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7316..7420)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7446..7901)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7583..7604)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7795..7814
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8028..8050
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8042..8059
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8043..8059
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8069..8088
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8069..8087
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        8158..8461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8463..8661
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8616..8636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8679..8859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8906..9031
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9528..9761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9946..9990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10288..10405
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10876,1..1181)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaagtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt ggaagcttgg cgcgccacca
     1201 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1261 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1321 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1381 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1441 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1501 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1561 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1621 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1681 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1741 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1801 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1861 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg
     1921 gcgggtccgg aggagagggc agaggaagtc tcctaacatg cggtgacgtg gaggagaatc
     1981 ctggcccaat gccgcgaagg aagaggaatg caggcagtag ttcagatgga accgaagatt
     2041 ccgatttttc tacagatctc gagcacacag acagttcaga aagtgatggc acatcccgac
     2101 gatctgctcg agtcacccgc tcctcagcca ggctaagcca gagttctcaa gattccagtc
     2161 ctgttcgaaa tctgcagtct tttggcactg aggagcctgc ttactctacc agaagagtga
     2221 cccgtagtca gcagcagcct accccagtga caccgaaaaa ataccctctt cggcagactc
     2281 gttcatctgg ttcagaaact gagcaagtgg ttgatttttc agatagagaa actaaaaata
     2341 cagctgatca tgatgagtca ccgcctcgaa ctccaactgg aaatgcgcct tcttctgagt
     2401 ctgacataga catctccagc cccaatgtat ctcacgatga gagcattgcc aaggacatgt
     2461 ccctgaagga ctcaggcagt gatctctctc atcgccccaa gcgccgtcgc ttccatgaaa
     2521 gctacaactt caatatgaag tgtcctacac caggctgtaa ctctctagga caccttacag
     2581 gaaaacatga gagacatttc tccatctcag gatgcccact gtatcataac ctctcagctg
     2641 acgaatgcaa ggtgagagca cagagccggg ataagcagat agaagaaagg atgctgtctc
     2701 acaggcaaga tgacaacaac aggcatgcaa ccaggcacca ggcaccaacg gagagacagc
     2761 ttcgatataa ggaaaaagtg gctgaactca ggaagaaaag aaattctgga ctgagcaaag
     2821 aacagaaaga gaaatatatg gaacacagac agacctatgg gaacacacgg gaacctcttt
     2881 tagaaaacct gacaagcgag tatgacttgg atcttttccg aagagcacaa gcccgggctt
     2941 cagaggattt ggagaagtta aggctgcaag gccaaatcac agagggaagc aacatgatta
     3001 aaacaattgc ttttggccgc tacgagctgg acacatggta tcattctcca tatcctgaag
     3061 aatatgcacg gctgggacgt ctctatatgt gtgaattctg tttaaaatat atgaagagcc
     3121 aaacgatact ccgccggcac atggccaaat gtgtgtggaa acacccacct ggtgatgaga
     3181 tatatcgcaa aggttcaatc tctgtgtttg aagtggatgg caagaaaaac aagatctact
     3241 gccaaaacct gtgcctgttg gccaaacttt ttctggacca caagacatta tattatgatg
     3301 tggagccctt cctgttctat gttatgacag aggcggacaa cactggctgt cacctgattg
     3361 gatatttttc taaggaaaag aattcattcc tcaactacaa cgtctcctgt atccttacta
     3421 tgcctcagta catgagacag ggctatggca agatgcttat tgatttcagt tatttgcttt
     3481 ccaaagtcga agaaaaagtt ggctccccag aacgtccact ctcagatctg gggcttataa
     3541 gctatcgcag ttactggaaa gaagtacttc tccgctatct ccacaacttc caaggcaaag
     3601 agatttctat caaagaaatc agtcaggaga cggctgtgaa tcctgtggac attgtcagca
     3661 ctctgcaagc ccttcagatg ctcaaatact ggaagggaaa acacctagtt ttaaagagac
     3721 aggacctgat tgatgagtgg atagccaaag aggccaaaag gtccaactcc aataaaacca
     3781 tggatcccag ctgcttaaaa tggacccctc ccaagggcac tggaagcgga gctggcgcta
     3841 aggagaagag tgcttgtcct aaagatccag ccaaacctcc ggccaaggca caagttgtgg
     3901 gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa aaatcaagcg
     3961 gtggcccgga ggcggcggcg ttcgtgaagg tatcaatgga cggagcaccg tacttgagga
     4021 aaatcgattt gaggatgtat aaataaaagt aagaattcgc ggccgctcaa cctctggatt
     4081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4441 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4621 cgagtcggat ctccctttgg gccgcctccc cgcaggtacc tttaagacca atgacttaca
     4681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4741 actcccaaag aagtcaagat ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     4801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4981 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5041 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5101 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5161 ggcaggacag caagggggag gattgggaag tcaatagcag gcatgctggg gatgcggtgg
     5221 gctctatggg cggccgttaa ttaagctttt gttcccttta gtgagggtta attgcgcgct
     5281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5341 acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7441 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7501 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7561 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7621 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7681 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7741 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7801 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7861 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     7921 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7981 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8041 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgactag
     8101 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     8161 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8221 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     8281 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     8341 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     8401 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     8461 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     8521 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     8581 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     8641 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     8701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8881 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8941 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9001 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9061 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9121 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     9181 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9241 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9301 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9361 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9421 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9481 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9541 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9601 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9661 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9721 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9781 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9841 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9901 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9961 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10021 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10081 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10141 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    10201 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
    10261 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
    10321 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    10381 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
    10441 gttagtaccg ggccctacgc gtgaggggcg cggaagccgg ggcggaggag gagccaaccc
    10501 cgggctgggc tgagacccgc agaggaagac gctctaggga tttgtcccgg actagcgaga
    10561 tggcaaggct gaggacggga ggctgattga gaggcgaagg tacaccctaa tctcaataca
    10621 acctttggag ctaagccagc aatggtagag ggaagattct gcacgtccct tccaggcggc
    10681 ctccccgtca ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa
    10741 ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa
    10801 cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga
    10861 gaagagcatg cgtgag
//
