LOCUS       sequence-31360-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK1 (3L19)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(756..774)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      770..817
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca aagaatcagg tattgagcta ccaagccttg
      121 ctaatgctat tgagaatatg cgtaaattcc agaactccca aaagcttgcg caggcagctc
      181 tactttatat ggcttcaaaa ctaacttctc aagaggaaac aaaagagcta acagatattt
      241 ttagacatat agacaaaaat ggcgatgggc agcttgatcg tcaagaacta attgatgggt
      301 attcaaagtt atctggagaa gaggttgcag tatttgattt acctcagatt gaaagtgaag
      361 tagatgcaat tctaggcgct gcagactttg atagaaatgg ttacatcgac tattcagagt
      421 ttgttactgt tgcaatggat cgaaaatctt tattaagcaa agataagttg gaatcagctt
      481 tccaaaaatt cgatcaagac gggaatggta agataagtgt agatgaactt gcttcagttt
      541 ttggattaga tcatttggaa agtaaaacat ggaaagaaat gatatctgga atagattcaa
      601 acaatgatgg cgatgttgat tttgaggaat tctgtaaaat gatacagaag ctctgttcta
      661 ataatgaacc ccagctctga tgacgaagct tgcggccgca ctcgaggatc cggctgctaa
      721 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      781 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaagggagga actatatccc
      841 ggatatcccg caagagggcc cggcagtacc ggcataacca g
//
