LOCUS       sequence-313611-        9753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y
  TITLE     Engineered Prime Editors with PAM flexibility.
  JOURNAL   Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022.
  PUBMED    33636398
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022."
COMMENT     Sequence Label: pCMV-PE2-VQR
FEATURES             Location/Qualifiers
     source          1..9753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             476..496
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             505..522
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(545..562)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    551..775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(846..862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(846..862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(859..881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    870..886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(894..900,901..918,919..924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    939..960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1077..1094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1248..1836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1328..1347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2007..2798,2799..2867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2630..2649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2868..2972)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3051..3070)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3242..3621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3622..3825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3776..3796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3867..3886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3867..3885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3935..3955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3956..8056
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gcgtaaggcg ggagctgcgg tgaccaccga gaccgaggta atctgggcta aagccctgcc
       61 agccgggaca tccgctcagc gggctgaact gatagcactc acccaggccc taaagatggc
      121 agaaggtaag aagctaaatg tttatactga tagccgttat gcttttgcta ctgcccatat
      181 ccatggagaa atatacagaa ggcgtgggtg gctcacatca gaaggcaaag agatcaaaaa
      241 taaagacgag atcttggccc tactaaaagc cctctttctg cccaaaagac ttagcataat
      301 ccattgtcca ggacatcaaa agggacacag cgccgaggct agaggcaacc ggatggctga
      361 ccaagcggcc cgaaaggcag ccatcacaga gactccagac acctctaccc tcctcataga
      421 aaattcatca ccctctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa
      481 gaagaagagg aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga
      541 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      601 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga gaaaattgca
      661 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      721 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
      781 gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa
      841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      901 cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta
      961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1861 gggtctgaca ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3001 gtcgacggat cgggagatcg atctcccgat cccctagggt ctactctcag tacaatctgc
     3061 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3241 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     3421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     3541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     3601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     3661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     3721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     3781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     3841 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
     3901 gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacaa
     3961 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
     4021 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     4081 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     4141 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     4201 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     4261 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     4321 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     4381 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     4441 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     4501 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     4561 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     4621 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     4681 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     4741 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     4801 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     4861 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     4921 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     4981 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     5041 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     5101 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     5161 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     5221 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     5281 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     5341 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     5401 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     5461 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     5521 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     5581 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     5641 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     5701 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     5761 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     5821 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     5881 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     5941 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     6001 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     6061 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     6121 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     6181 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     6241 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     6301 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     6361 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     6421 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     6481 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     6541 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     6601 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     6661 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     6721 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     6781 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     6841 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     6901 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     6961 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     7021 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     7081 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     7141 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     7201 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     7261 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     7321 gaaggactgg gaccctaaga agtacggcgg cttcgtgagc cccaccgtgg cctattctgt
     7381 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     7441 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     7501 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     7561 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     7621 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     7681 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     7741 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     7801 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     7861 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     7921 cttcaagtac tttgacacca ccatcgaccg gaagcagtac agaagcacca aagaggtgct
     7981 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     8041 tcagctggga ggtgactctg gaggatctag cggaggatcc tctggcagcg agacaccagg
     8101 aacaagcgag tcagcaacac cagagagcag tggcggcagc agcggcggca gcagcaccct
     8161 aaatatagaa gatgagtatc ggctacatga gacctcaaaa gagccagatg tttctctagg
     8221 gtccacatgg ctgtctgatt ttcctcaggc ctgggcggaa accgggggca tgggactggc
     8281 agttcgccaa gctcctctga tcatacctct gaaagcaacc tctacccccg tgtccataaa
     8341 acaatacccc atgtcacaag aagccagact ggggatcaag ccccacatac agagactgtt
     8401 ggaccaggga atactggtac cctgccagtc cccctggaac acgcccctgc tacccgttaa
     8461 gaaaccaggg actaatgatt ataggcctgt ccaggatctg agagaagtca acaagcgggt
     8521 ggaagacatc caccccaccg tgcccaaccc ttacaacctc ttgagcgggc tcccaccgtc
     8581 ccaccagtgg tacactgtgc ttgatttaaa ggatgccttt ttctgcctga gactccaccc
     8641 caccagtcag cctctcttcg cctttgagtg gagagatcca gagatgggaa tctcaggaca
     8701 attgacctgg accagactcc cacagggttt caaaaacagt cccaccctgt ttaatgaggc
     8761 actgcacaga gacctagcag acttccggat ccagcaccca gacttgatcc tgctacagta
     8821 cgtggatgac ttactgctgg ccgccacttc tgagctagac tgccaacaag gtactcgggc
     8881 cctgttacaa accctaggga acctcgggta tcgggcctcg gccaagaaag cccaaatttg
     8941 ccagaaacag gtcaagtatc tggggtatct tctaaaagag ggtcagagat ggctgactga
     9001 ggccagaaaa gagactgtga tggggcagcc tactccgaag acccctcgac aactaaggga
     9061 gttcctaggg aaggcaggct tctgtcgcct cttcatccct gggtttgcag aaatggcagc
     9121 ccccctgtac cctctcacca aaccggggac tctgtttaat tggggcccag accaacaaaa
     9181 ggcctatcaa gaaatcaagc aagctcttct aactgcccca gccctggggt tgccagattt
     9241 gactaagccc tttgaactct ttgtcgacga gaagcagggc tacgccaaag gtgtcctaac
     9301 gcaaaaactg ggaccttggc gtcggccggt ggcctacctg tccaaaaagc tagacccagt
     9361 agcagctggg tggccccctt gcctacggat ggtagcagcc attgccgtac tgacaaagga
     9421 tgcaggcaag ctaaccatgg gacagccact agtcattctg gccccccatg cagtagaggc
     9481 actagtcaaa caaccccccg accgctggct ttccaacgcc cggatgactc actatcaggc
     9541 cttgcttttg gacacggacc gggtccagtt cggaccggtg gtagccctga acccggctac
     9601 gctgctccca ctgcctgagg aagggctgca acacaactgc cttgatatcc tggccgaagc
     9661 ccacggaacc cgacccgacc taacggacca gccgctccca gacgccgacc acacctggta
     9721 cacggatgga agcagtctct tacaagaggg aca
//
