LOCUS       sequence-313613-        1911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pVD_85
FEATURES             Location/Qualifiers
     source          1..1911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..166)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     87..111
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        215..621
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             652..672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagcgacctc atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga
       61 ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt
      121 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt
      181 caacaacgta tctaccaacg gaatgcgtgc gatgcacaca ccatagcttc aaaatgtttc
      241 tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta ccacttcaaa
      301 acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt cgttaattac
      361 ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa
      421 aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt ttgatttttt
      481 tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt
      541 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
      601 agcatagcaa tctaatctaa gttttaatta caaatctgtc ataaaacaat gcccaagaag
      661 aaaaggaaag ttaatcaacc agtagaaaga cagcgtagga gaactactca aagtgctaca
      721 attcgtgacg tagctgcaag agcaggtgtc tctcctatga cagtctcacg tgtaatcaat
      781 agagagtcca cagttaaaga ggaaactaga cagttggttg aaaaggcaat agccgacctt
      841 aactatgctc ctaatcctgc agccagatct ttggcaggta gtgccccttt tagaattggc
      901 ttactgtacg ataatccttc aactggctac ctttctgaat ttctagttgg tgccttagat
      961 gaatcaagta gaaccggtgc tcaaattgtt atcgagaaat gtgctgaacc agaattagcc
     1021 agagctacac ttgctagatt gttgaaaact ggagttgatg gacttatctt acctccacca
     1081 ttatgcgaat ctccagaagt tctggccgag ataagagccg caggagctgc cgctgtcgca
     1141 gtggcacctg gtacagcttc tgccgacatg gctactatta gaatcgacaa cgaagcagct
     1201 gcatttgagt tgacccagca tttgattggc ttgggtcaca aaagattcgg attcattaag
     1261 ggtcatccaa atcaaaccgt gtctcaacaa aggcttgatg ggtttatgac tgctcttaag
     1321 gctgcaggga tcccacaaga gaatatcaga gtggaacaag gttacttcac atatcgttca
     1381 ggtctagaag ctgcagagag actactagca gccgaaccta ggccaactgc catcttcgct
     1441 gctaacgatg atatggcagc tgcaacagca ggcgtagcac atagactagg cttggatgta
     1501 ccaggcgacg tgtctatagt gggatttgat gatacttcca tagctgataa catttggcca
     1561 ccattaacaa cagttcacca accaattgcc gctatggcca gagccgctgt tgatctggtt
     1621 ctagaagaga tcagaaggca tagagatggt ggtggcgaac ctagacaatt gatgcatcca
     1681 cacactctga tcgttagaga ctcctcaggc cctgctggag tctaataatc gcgtgcattc
     1741 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     1801 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     1861 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga a
//
