LOCUS       sequence-313615-        9753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y
  TITLE     Engineered Prime Editors with PAM flexibility.
  JOURNAL   Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022.
  PUBMED    33636398
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 
            10.1016/j.ymthe.2021.02.022."
COMMENT     Sequence Label: pCMV-PE2-VRER
FEATURES             Location/Qualifiers
     source          1..9753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..35)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(189..777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(269..288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(948..1739,1740..1808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1571..1590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1809..1913)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1992..2011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2183..2562
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2563..2766
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2717..2737
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2808..2827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2808..2826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2876..2896
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2897..6997
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKEYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9170..9190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9199..9216
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9239..9256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9245..9469
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9540..9556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9540..9556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9553..9575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9564..9580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9588..9594,9595..9612,9613..9618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9633..9654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
       61 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      781 gatcctttga tcttttctac ggggtctgac actcagtgga acgaaaactc acgttaaggg
      841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1921 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg
     1981 tctactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     2041 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     2101 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     2161 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     2221 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2281 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2341 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2401 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2461 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2521 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2581 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2641 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2701 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2761 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
     2821 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
     2881 gaagaagcgg aaagtcgaca agaagtacag catcggcctg gacatcggca ccaactctgt
     2941 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     3001 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     3061 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     3121 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     3181 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     3241 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     3301 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     3361 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     3421 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     3481 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     3541 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     3601 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     3661 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     3721 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     3781 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     3841 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     3901 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     3961 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     4021 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     4081 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     4141 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     4201 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     4261 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     4321 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     4381 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     4441 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     4501 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     4561 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     4621 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     4681 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     4741 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     4801 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     4861 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     4921 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     4981 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     5041 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     5101 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     5161 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     5221 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     5281 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     5341 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     5401 cgatgtggac gctatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     5461 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     5521 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     5581 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     5641 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     5701 ctcccgcatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     5761 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     5821 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     5881 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     5941 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     6001 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     6061 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     6121 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     6181 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     6241 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgtgag
     6301 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     6361 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     6421 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     6481 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     6541 tgccagagaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     6601 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     6661 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     6721 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     6781 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     6841 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaaggagta
     6901 cagaagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     6961 cgagacacgg atcgacctgt ctcagctggg aggtgactct ggaggatcta gcggaggatc
     7021 ctctggcagc gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag
     7081 cagcggcggc agcagcaccc taaatataga agatgagtat cggctacatg agacctcaaa
     7141 agagccagat gtttctctag ggtccacatg gctgtctgat tttcctcagg cctgggcgga
     7201 aaccgggggc atgggactgg cagttcgcca agctcctctg atcatacctc tgaaagcaac
     7261 ctctaccccc gtgtccataa aacaataccc catgtcacaa gaagccagac tggggatcaa
     7321 gccccacata cagagactgt tggaccaggg aatactggta ccctgccagt ccccctggaa
     7381 cacgcccctg ctacccgtta agaaaccagg gactaatgat tataggcctg tccaggatct
     7441 gagagaagtc aacaagcggg tggaagacat ccaccccacc gtgcccaacc cttacaacct
     7501 cttgagcggg ctcccaccgt cccaccagtg gtacactgtg cttgatttaa aggatgcctt
     7561 tttctgcctg agactccacc ccaccagtca gcctctcttc gcctttgagt ggagagatcc
     7621 agagatggga atctcaggac aattgacctg gaccagactc ccacagggtt tcaaaaacag
     7681 tcccaccctg tttaatgagg cactgcacag agacctagca gacttccgga tccagcaccc
     7741 agacttgatc ctgctacagt acgtggatga cttactgctg gccgccactt ctgagctaga
     7801 ctgccaacaa ggtactcggg ccctgttaca aaccctaggg aacctcgggt atcgggcctc
     7861 ggccaagaaa gcccaaattt gccagaaaca ggtcaagtat ctggggtatc ttctaaaaga
     7921 gggtcagaga tggctgactg aggccagaaa agagactgtg atggggcagc ctactccgaa
     7981 gacccctcga caactaaggg agttcctagg gaaggcaggc ttctgtcgcc tcttcatccc
     8041 tgggtttgca gaaatggcag cccccctgta ccctctcacc aaaccgggga ctctgtttaa
     8101 ttggggccca gaccaacaaa aggcctatca agaaatcaag caagctcttc taactgcccc
     8161 agccctgggg ttgccagatt tgactaagcc ctttgaactc tttgtcgacg agaagcaggg
     8221 ctacgccaaa ggtgtcctaa cgcaaaaact gggaccttgg cgtcggccgg tggcctacct
     8281 gtccaaaaag ctagacccag tagcagctgg gtggccccct tgcctacgga tggtagcagc
     8341 cattgccgta ctgacaaagg atgcaggcaa gctaaccatg ggacagccac tagtcattct
     8401 ggccccccat gcagtagagg cactagtcaa acaacccccc gaccgctggc tttccaacgc
     8461 ccggatgact cactatcagg ccttgctttt ggacacggac cgggtccagt tcggaccggt
     8521 ggtagccctg aacccggcta cgctgctccc actgcctgag gaagggctgc aacacaactg
     8581 ccttgatatc ctggccgaag cccacggaac ccgacccgac ctaacggacc agccgctccc
     8641 agacgccgac cacacctggt acacggatgg aagcagtctc ttacaagagg gacagcgtaa
     8701 ggcgggagct gcggtgacca ccgagaccga ggtaatctgg gctaaagccc tgccagccgg
     8761 gacatccgct cagcgggctg aactgatagc actcacccag gccctaaaga tggcagaagg
     8821 taagaagcta aatgtttata ctgatagccg ttatgctttt gctactgccc atatccatgg
     8881 agaaatatac agaaggcgtg ggtggctcac atcagaaggc aaagagatca aaaataaaga
     8941 cgagatcttg gccctactaa aagccctctt tctgcccaaa agacttagca taatccattg
     9001 tccaggacat caaaagggac acagcgccga ggctagaggc aaccggatgg ctgaccaagc
     9061 ggcccgaaag gcagccatca cagagactcc agacacctct accctcctca tagaaaattc
     9121 atcaccctct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa
     9181 gaggaaagtc taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     9241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     9301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgagaaaat tgcatcgcat
     9361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     9421 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     9481 gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg
     9541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9601 ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg
     9661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9721 ggccaacgcg cggggagagg cggtttgcgt att
//
