LOCUS       sequence-313618-        1461 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pVD_79
FEATURES             Location/Qualifiers
     source          1..1461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..166)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     87..111
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        215..621
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gagcgacctc atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga
       61 ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt
      121 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt
      181 caacaacgta tctaccaacg gaatgcgtgc gatgcacaca ccatagcttc aaaatgtttc
      241 tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta ccacttcaaa
      301 acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt cgttaattac
      361 ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa
      421 aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt ttgatttttt
      481 tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt
      541 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
      601 agcatagcaa tctaatctaa gttttaatta caaatctgtc ataaaacaat gatgtctaga
      661 ctggacaaat ctaaggttat aaacagtgca ctagaattgt tgaatgaagt tggtatcgag
      721 ggtcttacta cgagaaagtt agcacaaaaa ttaggcgttg aacaacctac tctttattgg
      781 catgtcaaaa acaagcgtgc attgctggac gcactagcta tagaaatgtt ggacagacac
      841 cacacgcact tctgcccttt ggaaggtgag tcatggcagg atttcctaag aaacaacgca
      901 aagagcttta ggtgtgcatt gttgagtcat agggacggcg ctaaggtcca tctaggtact
      961 agaccaaccg aaaaacaata cgaaacactg gaaaaccaac tagcgttctt atgtcagcaa
     1021 ggtttttctt tggaaaatgc cttatatgca ctgtccgctg taggccattt tactctgggt
     1081 tgtgtcttag aagaccaaga acatcaagta gccaaggagg agagagaaac tcctaccacc
     1141 gattctatgc ctcccctttt acgtcaagcg atcgagctgt ttgatcatca aggggctgaa
     1201 cccgcctttc tttttggcct tgaattgata atttgcggtc tagagaaaca gttaaaatgc
     1261 gaatccggct cttagtaatc gcgtgcattc atccgctcta accgaaaagg aaggagttag
     1321 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
     1381 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
     1441 actgaaaacc ttgcttgaga a
//
