LOCUS       sequence-31362-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-GNPNAT1
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(605..623)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      619..666
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattcctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcgtg gacctggaac ttagggtgtt
      121 ggaggagagt gacttgtcca gtcacttgga gctgttgggg catctgactg aggcaccacc
      181 gttgagcggg gtcgaactcg cgaacatcgc ggacatgcgg aggagagctg gaattgtaac
      241 taaagtgttc tgtcaccaac caactggacg gattgtgggg tcagcatctc taatgattca
      301 accgaaattc actcgtggcg gtagggctgt aggacacatt gaagatgtcg ttgtagaccc
      361 ctcgtacaga ggggctggac ttggcaaggc acttatcatg gacctttgcg aaatatctag
      421 gtcgaaaggt tgctacaaag tgatactcga cagttctgaa aagtcgcttc cgttctacga
      481 aaagttgggg tttcgagcac atgagagaca aatgaggctg gatttgtgat gacgaagctt
      541 gcggccgcac tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      601 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      661 tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca
      721 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
      781 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
      841 attaaagctt tatcgatgat aagctgtcaa acatgagaat tctttgaaga cg
//
