LOCUS       sequence-313622-        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO
            -108 -yeGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pFA197
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             305..1021
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccaggcaact ttagtgctga cacatacagg catatatata tgtgtgcgac gacacatgat
       61 catatggcat gcatgtgctc tgtatgtata taaaactctt ggatcgtata acctcctggt
      121 taagggaaag ccacgaaata tcattttacc taaccggatg aaacatccaa atctgacgac
      181 gttttcttct tttctctaaa tattctttcc ttatacatta ggacctttgc agcataaatt
      241 actatacttc tatagacaca caaacacaaa tacacacact aaattaataa tctgtcataa
      301 aacaatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
      361 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
      421 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
      481 cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa
      541 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
      601 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
      661 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
      721 caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa
      781 tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc
      841 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
      901 ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
      961 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata
     1021 aatcgcgtgc attcatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     1081 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     1141 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     1201 gagaa
//
