LOCUS       sequence-31363-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-BRD1
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(498..518)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(531..548)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             558..580
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    595..619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(619..638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(620..638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(722..741)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcgggctttg ttagcagccg gatcctcgag tgcggccgca agcttcgtca tcaattattt
       61 tctaattcgt ctatttcctc tatttgatct tctttttctt tactccactt aatattttct
      121 tcttctacaa gttttaacat gtcattaatt tttttttctt ttaaaatgaa gttattatat
      181 cccattacta ttaactggtt attaaaataa gtttcgatat ttttgcccgt aattgcgact
      241 acagaatttg aagtattata taaactacag ttatcaaaaa ttaattgaac atcttttaca
      301 aattctgaag gatatgcata ctgacccttc tttaatttgg tttttataca actaaatgac
      361 attgggtttt taattacatc ataataatct ggacaattct gttttttagc atctactaat
      421 ttctcaaata tatgaccacc ttcatattta cttaaactta atattaattg attagctaat
      481 aaataccatt gcttattgcc ctggaaatac aagttttctc tgccgctgct gtgatgatga
      541 tgatgatgca tggtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
      601 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      661 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      721 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      781 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      841 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      901 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag at
//
