LOCUS       sequence-313632-        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - 
            pCYC1_BenO_182 -yeGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pFA272
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             298..1014
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccaggcaact ttagtgctga cacatacata ctccataggt attttattat acaaataatg
       61 tgtttgaact tattaaaaca ttcttttaag gtataaacaa aggcatatat atatgtgtgc
      121 gacgacacat gatcatatgg catgcatgtg ctctgtatgt atataaaact cttgttttct
      181 tcttttctct aaatattctt tccttataca ttaggacctt tgcagcataa attactatac
      241 ttctatagac acacaaacac aaatacacac actaaattaa taatctgtca taaaacaatg
      301 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
      361 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa
      421 ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc
      481 actactttcg gttatggtgt tcaatgtttt gcgagatacc cagatcatat gaaacaacat
      541 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
      601 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
      661 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
      721 gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc
      781 aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat
      841 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
      901 tccactcaat ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta
      961 gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa ataaatcgcg
     1021 tgcattcatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     1081 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     1141 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaa
//
