LOCUS       sequence-313637-       10331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti-viral construct with tamoxifen inducible Cre recombinase and 
            U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the 
            stuffer 1.8kb with BsmBI). NO Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Loganathan SK, Schleicher K, Malik A, Quevedo R, Langille E, Teng K,
            Oh RH, Rathod B, Tsai R, Samavarchi-Tehrani P, Pugh TJ, Gingras AC, 
            Schramek D
  TITLE     Rare driver mutations in head and neck squamous cell carcinomas 
            converge on NOTCH signaling.
  JOURNAL   Science. 2020 Mar 13;367(6483):1264-1269. doi: 
            10.1126/science.aax0902.
  PUBMED    32165588
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-03-13- 13"; volume: "367"; 
            issue: "6483"; pages: "1264-1269"
COMMENT     Sequence Label: pLKO-CreERT2 stuffer v3
FEATURES             Location/Qualifiers
     source          1..10331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1586..2521
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1634..2527
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     LTR             2599..2832
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2904..3025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2941..2960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2995..3014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3065..3200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3220..3239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3221..3239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3249..3266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3249..3265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3258..3280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3407..3862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3494..3513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3704..3725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3888..3992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3993..4061,4062..4853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4211..4230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5024..5612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5513..5532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5766..5783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5900..5921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5936..5941,5942..5959,5960..5966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5974..5990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5979..6001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5998..6014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5998..6014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6033..6053
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6035..6053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6081..6307
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6308..6488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6535..6660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7153..7386
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7571..7615
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7913..8161
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7913..7933
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8084..8103
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10043..10118
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10166..10283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca ggtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatccc gggtcgacat caagcttggc
      541 caccatgtcc aacctgctga ctgtgcacca aaacctgcct gccctccctg tggatgccac
      601 ctctgatgaa gtcaggaaga acctgatgga catgttcagg gacaggcagg ccttctctga
      661 acacacctgg aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa
      721 caacaggaaa tggttccctg ctgaacctga ggatgtgagg gactacctcc tgtacctgca
      781 agccagaggc ctggctgtga agaccatcca acagcacctg ggccagctca acatgctgca
      841 caggagatct ggcctgcctc gcccttctga ctccaatgct gtgtccctgg tgatgaggag
      901 aatcagaaag gagaatgtgg atgctgggga gagagccaag caggccctgg cctttgaacg
      961 cactgacttt gaccaagtca gatccctgat ggagaactct gacagatgcc aggacatcag
     1021 gaacctggcc ttcctgggca ttgcctacaa caccctgctg cgcattgccg aaattgccag
     1081 aatcagagtg aaggacatct cccgcaccga tggtgggaga atgctgatcc acattggcag
     1141 gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa
     1201 gctggtggag agatggatct ctgtgtctgg tgtggctgat gaccccaaca actacctgtt
     1261 ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc acctcccaac tgtccacccg
     1321 ggccctggaa gggatctttg aggccaccca ccgcctgatc tatggtgcca aggatgactc
     1381 tgggcagaga tacctggcct ggtctggcca ctctgccaga gtgggtgctg ccagggacat
     1441 ggccagggct ggtgtgtcca tccctgaaat catgcaggct ggtggctgga ccaatgtgaa
     1501 cattgtgatg aactacatca gaaacctgga ctctgagact ggggccatgg tgaggcttct
     1561 agaggatggg gacctcgagc catctgctgg agacatgaga gctgccaacc tttggccaag
     1621 cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca
     1681 gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac
     1741 cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct
     1801 ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga
     1861 tcaggtccac cttcttgaat gtgcctggct agagatcctg atgattggtc tcgtctggcg
     1921 ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca
     1981 gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg
     2041 gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct
     2101 taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca
     2161 tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg
     2221 cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat
     2281 caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt
     2341 gcccctctat gacctgctgc tggaggcggc ggacgcccac cgcctacatg cgcccactag
     2401 ccgtggaggg gcatccgtgg aggagaccga ccaaagccac ttggccactg cgggctctac
     2461 ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac
     2521 agcttgaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2581 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2641 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2701 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2761 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2821 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     2881 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     2941 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3001 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3061 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3181 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3241 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3841 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5821 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5881 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5941 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6001 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6061 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6121 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6181 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6241 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6301 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6361 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6421 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6481 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6541 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6601 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6661 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6721 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6781 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6841 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6901 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     6961 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7021 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7081 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7141 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7201 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7261 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7321 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7381 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7441 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     7501 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     7561 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     7621 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7681 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     7741 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     7801 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     7861 atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcccccttca ccgagggcct
     7921 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg
     7981 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     8041 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     8101 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     8161 cggagacggt tgtaaatgag cacacaaaat acacatgcta aaatattata ttctatgacc
     8221 tttataaaat caaccaaaat cttcttttta ataactttag tatcaataat tagaattttt
     8281 atgttccttt ttgcaaactt ttaataaaaa tgagcaaaat aaaaaaacgc tagttttagt
     8341 aactcgcgtt gttttcttca cctttaataa tagctactcc accacttgtt cctaagcggt
     8401 cagctcctgc ttcaatcatt ttttgagcat cttcaaatgt tctaactcca ccagctgctt
     8461 taactaaagc attgtcttta acaactgact tcattagttt aacatcttca aatgttgcac
     8521 ctgattttga aaatcctgtt gatgttttaa caaattctaa tccagcttca acagctattt
     8581 cacaagcttt catgatttct tcttttgtta ataaacaatt ttccataata catttaacaa
     8641 catgtgatcc agctgctttt tttacagctt tcatgtcttc taaaactaat tcataatttt
     8701 tgtcttttaa tgcaccaata tttaatacca tatcaatttc tgttgcacca tctttaattg
     8761 cttcagaaac ttcgaatgct tttgtagctg ttgtgcatgc acctagagga aaacctacaa
     8821 catttgttat tcctacattt gtgcctttta ataattcttt acaatagctt gttcaatatg
     8881 aattaacaca aactgttgca aaatcaaatt caattgcttc atcacataat tgtttaattt
     8941 cagctttcgt agcatcttgt tttaataatg tgtgatctat atatttgttt agtttcattt
     9001 tttctcctat atattcattt ttaattttaa ttctttaata atttcgtcta ctttaacttt
     9061 agcgttttga acagattcac caacacctat aaaataaatt tttagtttag gttcagttcc
     9121 acttgggcga acagcaaatc atgacttatc ttctaaataa aattttagta agtcttgtcc
     9181 tggcatatta tacattccat cgatgtagtc ttcaacatta acaactttaa gtccagcaat
     9241 ttgagttaag ggtgttgctc tcaatgattt cattaatggt tcaattttta atttcttttc
     9301 ttctggttta aaattcaagt ttaaagtgaa agtgtaatat gcacccattt ctttaaataa
     9361 atcttctaaa tagtctacta atgttttatt ttgtttttta taaaatcaag cagcctctgc
     9421 tattaatata gaagcttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc
     9481 ataactttct tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc
     9541 tctacccatt catttaaatc cagttaaagt ttttacaata ttaactccat atttttcatg
     9601 agcgattcta tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg
     9661 aatgctattt aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg
     9721 atttccatct aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc
     9781 atctgggtca ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat
     9841 ttttcatgca ggatcaaatt ctggatttgg atttacaaca tttttaaatg tttcatcttc
     9901 aaatgcatgc tcttcaacct caataacgtt atatcctgat tcacgtaata tttttggggt
     9961 aaatttagtt cctgttccat taactgcgct aaaaataatt tttaaatctt ttttagcttc
    10021 ttgctctttt ttgtacgtct ctgttttaga gctagaaata gcaagttaaa ataaggctag
    10081 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc tcgacctcga
    10141 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10201 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10261 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10321 cgcggctcga g
//
