LOCUS       sequence-313640-        8471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti-viral construct with tamoxifen inducible Cre recombinase and 
            U6 driven sgRNA against mouse Ripk4. NO Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Loganathan SK, Schleicher K, Malik A, Quevedo R, Langille E, Teng K,
            Oh RH, Rathod B, Tsai R, Samavarchi-Tehrani P, Pugh TJ, Gingras AC, 
            Schramek D
  TITLE     Rare driver mutations in head and neck squamous cell carcinomas 
            converge on NOTCH signaling.
  JOURNAL   Science. 2020 Mar 13;367(6483):1264-1269. doi: 
            10.1126/science.aax0902.
  PUBMED    32165588
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-03-13- 13"; volume: "367"; 
            issue: "6483"; pages: "1264-1269"
COMMENT     Sequence Label: pLKO-CreERT2 sgRipk4 v3
FEATURES             Location/Qualifiers
     source          1..8471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    233..354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(270..289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     324..343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      394..529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(549..568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(578..595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(578..594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(587..609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      736..1191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(823..842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1033..1054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1217..1321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1322..1390,1391..2182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1540..1559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2353..2941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2842..2861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3095..3112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3229..3250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3265..3270,3271..3288,3289..3295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3303..3319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3308..3330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3327..3343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3327..3343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3362..3382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3364..3382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3410..3636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3637..3817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3864..3989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4482..4715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4900..4944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5242..5490
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5242..5262
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5413..5432
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5512..5587
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5635..5752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5801..6311
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6230..6248
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7386..8321
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             7434..8327
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     LTR             join(8399..8471,1..161)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
       61 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      121 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
      181 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
      241 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      301 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
      361 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      421 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      481 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
      541 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
      601 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      661 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      721 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      781 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      841 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      901 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      961 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1021 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     1081 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1141 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     1201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1561 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1861 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2041 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3001 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3061 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     3241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     3301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     3361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     3421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     3481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     3541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     3601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     3661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     3841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     3901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     3961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     4021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     4081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     4141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     4201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     4261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     4321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     4981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct
     5221 cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata
     5281 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     5341 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     5401 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     5461 ttatatatct tgtggaaagg acgaaacacc gcgtgcacga tgcgaaagcg cgttttagag
     5521 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     5581 tcggtgcttt tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     5641 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     5701 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     5761 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
     5821 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     5881 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     5941 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     6001 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca ggtctcacta
     6061 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     6121 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     6181 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     6241 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     6301 tctctcccca gggggatccc gggtcgacat caagcttggc caccatgtcc aacctgctga
     6361 ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga
     6421 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     6481 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa caacaggaaa tggttccctg
     6541 ctgaacctga ggatgtgagg gactacctcc tgtacctgca agccagaggc ctggctgtga
     6601 agaccatcca acagcacctg ggccagctca acatgctgca caggagatct ggcctgcctc
     6661 gcccttctga ctccaatgct gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg
     6721 atgctgggga gagagccaag caggccctgg cctttgaacg cactgacttt gaccaagtca
     6781 gatccctgat ggagaactct gacagatgcc aggacatcag gaacctggcc ttcctgggca
     6841 ttgcctacaa caccctgctg cgcattgccg aaattgccag aatcagagtg aaggacatct
     6901 cccgcaccga tggtgggaga atgctgatcc acattggcag gaccaagacc ctggtgtcca
     6961 cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa gctggtggag agatggatct
     7021 ctgtgtctgg tgtggctgat gaccccaaca actacctgtt ctgccgggtc agaaagaatg
     7081 gtgtggctgc cccttctgcc acctcccaac tgtccacccg ggccctggaa gggatctttg
     7141 aggccaccca ccgcctgatc tatggtgcca aggatgactc tgggcagaga tacctggcct
     7201 ggtctggcca ctctgccaga gtgggtgctg ccagggacat ggccagggct ggtgtgtcca
     7261 tccctgaaat catgcaggct ggtggctgga ccaatgtgaa cattgtgatg aactacatca
     7321 gaaacctgga ctctgagact ggggccatgg tgaggcttct agaggatggg gacctcgagc
     7381 catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct
     7441 ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg
     7501 atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt
     7561 cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg
     7621 cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac cttcttgaat
     7681 gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga
     7741 agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca
     7801 tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc
     7861 agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat
     7921 ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca
     7981 agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc
     8041 accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag
     8101 gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc
     8161 tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg
     8221 aggagaccga ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa
     8281 agtattacat cacgggggag gcagagggtt tccctgccac agcttgaggt acctttaaga
     8341 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8401 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8461 tggttagacc a
//
