LOCUS       sequence-313663-        4380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133803
FEATURES             Location/Qualifiers
     source          1..4380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             712..732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             733..774
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    827..948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(838..857)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     892..911
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1063..1109
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1139..1275
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1320..1391
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1617..2205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1697..1716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2951..3019,3020..3811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3169..3188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3824..3960
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4112..4183
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4188..4234)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4252..4300
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgataga actatcgctc
      601 atcgactttt atctgtgctt cttggctttc ctgctttttc tggtgctcat tatgctaatc
      661 attttttggt tcagtctgga gttacaagat cataatgaga catgtcacgc cgaaaacctt
      721 tactttcagt cggggaaacc tatacccaat ccacttctcg gcttagattc tacctaagct
      781 agcttgactg actgagatac agcgtacctt cagctcacag acatgataag atacattgat
      841 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      901 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      961 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     1021 aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg
     1081 cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc
     1141 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
     1201 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
     1261 gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac
     1321 attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca tcaaactttc
     1381 ttccctccct aatctcattg gttaccttgg gctatcgaaa cttaattaac cagtcaagtc
     1441 agctacttgg cgagatcgac ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa
     1501 gttcgatctg gtccttgcta ttgcacccgt tctccgatta cgagtttcat ttaaatcatg
     1561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2401 cctatctcag cgatctgtct atttcgttca tccatagttg catttaaatt tccgaactct
     2461 ccaaggccct cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc aggctacctc
     2521 tcgaacgaac tatcgcaagt ctcttggccg gccttgcgcc ttggctattg cttggcagcg
     2581 cctatcgcca ggtattactc caatcccgaa tatccgagat cgggatcacc cgagagaagt
     2641 tcaacctaca tcctcaatcc cgatctatcc gagatccgag gaatatcgaa atcggggcgc
     2701 gcctggtgta ccgagaacga tcctctcagt gcgagtctcg acgatccata tcgttgcttg
     2761 gcagtcagcc agtcggaatc cagcttggga cccaggaagt ccaatcgtca gatattgtac
     2821 tcaagcctgg tcacggcagc gtaccgatct gtttaaacct agatattgat agtctgatcg
     2881 gtcaacgtat aatcgagtcc tagcttttgc aaacatctat caagagacag gatcagcagg
     2941 aggctttcgc atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3001 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3061 gttgggtgcg cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3121 ttttcgcccc gaagaacgct ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3181 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3241 gaatgacttg gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt
     3301 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3361 gacaacgatt ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3421 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3481 caccacgatg cctgtagcaa tggcaacaac cttgcgtaaa ctattaactg gcgaactact
     3541 tactctagct tcccggcaac agttgataga ctggatggag gcggataaag ttgcaggacc
     3601 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3661 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3721 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3781 gataggtgcc tcactgatta agcattggta accgattcta ggtgcattgg cgcagaaaaa
     3841 aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc agcaacggat
     3901 acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat ttatccttaa
     3961 gatcccgaat cgtttaaact cgactctggc tctatcgaat ctccgtcgtt tcgagcttac
     4021 gcgaacagcc gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc tatcgtcagc
     4081 ttaccttttt ggcagcgatc gcggctcccg acatcttgga ccattagctc cacaggtatc
     4141 ttcttccctc tagtggtcat aacagcagct tcagctacct ctcaattcaa aaaacccctc
     4201 aagacccgtt tagaggcccc aaggggttat gctatcaatc gttgcgttac acacacaaaa
     4261 aaccaacaca catccatctt cgatggatag cgattttatt atctaactgc tgatcgagtg
     4321 tagccagatc tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
