LOCUS       sequence-313665-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133859
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             625..645
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             646..687
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    740..861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(751..770)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     805..824
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      976..1022
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1052..1188
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1233..1304
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1530..2118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1610..1629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2864..2932,2933..3724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3082..3101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3737..3873
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4025..4096
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4101..4147)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4165..4213
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgagtgc cgatgctcag
      601 tcctttctca acggtttcgc agtggaaaac ctttactttc agtcggggaa acctataccc
      661 aatccacttc tcggcttaga ttctacctaa gctagcttga ctgactgaga tacagcgtac
      721 cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      781 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      841 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      901 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca
      961 tctctatcgg tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     1021 tgtgcccctc gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc
     1081 gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca
     1141 acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta
     1201 tcctgcaggc gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac
     1261 cactaggggt cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct
     1321 tgggctatcg aaacttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg
     1381 ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc
     1441 cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg
     1501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2341 tcatccatag ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa
     2401 acctttcgtc cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg
     2461 ccggccttgc gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc
     2521 gaatatccga gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta
     2581 tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc
     2641 agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg
     2701 ggacccagga agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga
     2761 tctgtttaaa cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt
     2821 tgcaaacatc tatcaagaga caggatcagc aggaggcttt cgcatgagta ttcaacattt
     2881 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2941 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg gttacatcga
     3001 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gctttccaat
     3061 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3121 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     3181 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3241 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     3301 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3361 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3421 aaccttgcgt aaactattaa ctggcgaact acttactcta gcttcccggc aacagttgat
     3481 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3541 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3601 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3661 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3721 gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
     3781 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
     3841 tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct
     3901 ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc
     3961 gtcgggcatc gaatctcgtc agctatcgtc agcttacctt tttggcagcg atcgcggctc
     4021 ccgacatctt ggaccattag ctccacaggt atcttcttcc ctctagtggt cataacagca
     4081 gcttcagcta cctctcaatt caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
     4141 tatgctatca atcgttgcgt tacacacaca aaaaaccaac acacatccat cttcgatgga
     4201 tagcgatttt attatctaac tgctgatcga gtgtagccag atctagtaat caattacggg
     4261 gtcattagtt catagcccat atatggagtt ccg
//
