LOCUS       sequence-313670-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pSB700
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..579
                     /label=chicken beta-actin promoter
     intron          580..1596
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1520..1542
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1604..1623
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1624..1633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1630..1632,1633..1635,1636..2349)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1675..1696)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1936..1955)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2283..2304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2362..2950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2415..2435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2833..2844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3157..3790
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3919..3935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3919..3935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3932..3954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3943..3959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3967..3973,3974..3991,3992..3997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4012..4033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4150..4167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4321..4909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4401..4420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5080..5871,5872..5940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5703..5722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5941..6045)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6093..6227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6130..6149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6184..6203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6395..7028
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7075..7200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7697..7930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8115..8159
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8422..8537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8640..8888
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8640..8660
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8811..8830
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8910..8985
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggcgcgcgc caggcggggc ggggcggggs grggggsggg gsggggcgag gcggagaggt
      481 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      541 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      601 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      661 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      721 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      781 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      841 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      901 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      961 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     1021 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     1081 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     1141 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1201 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     1261 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1321 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1381 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1441 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1501 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1561 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1621 gtggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1681 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1741 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1801 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     1861 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1921 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1981 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2041 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     2101 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     2161 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2221 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     2281 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2341 tacaagtaaa gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     2401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     2761 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2881 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2941 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     3001 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     3061 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     3121 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     3181 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     3241 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     3301 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     3361 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     3421 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     3481 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     3541 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     3601 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3781 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     3841 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
     3901 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6061 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     6121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     6241 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     6301 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     6361 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     6421 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     6481 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     6541 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     6601 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     6661 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     6721 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     6781 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     6841 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7021 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7081 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7141 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7201 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7261 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7321 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7381 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7441 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7501 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     7561 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     7621 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     7681 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     7741 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7801 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7861 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     7921 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7981 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8041 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8101 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8161 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     8221 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     8281 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     8341 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     8401 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     8461 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     8521 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcattag
     8581 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg
     8641 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     8701 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     8761 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     8821 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     8881 gaaacaccgg agacgattaa tgcgtctcgg ttttagagct agaaatagca agttaaaata
     8941 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgaattctc
     9001 gacctcgaga caaatggcag tattcgtcat tagttcatag cccatatatg gagttccg
//
