LOCUS       sequence-31369-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-ChK
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcaa attaacagat cctttatata
      121 ttaaaaaaat atgtcttgaa aaagtacatg attggtctag gtgtaatgaa gatgatgtat
      181 gtgtaaatca aattttaagt ggattaacta atcaactttt tgaagttagt ataaaagaag
      241 acacagcaat tgaatataga ataacgagaa gacatgtttt atttagaata tatggaaaag
      301 atgtagatgc attatataat cctttaagtg aatttgaagt atataaaaca atgagtaaat
      361 atagaatagc acctttatta ttaaatacat ttgatggtgg gcgtatcgag gaatggttat
      421 atggagatcc tctaagtatt gatgatttaa aaaataaatc tatattagtt ggaatagcca
      481 atgtattagg aaaatttcat acacttagtc gaaaacgtca tttacctgaa cactgggata
      541 aaactccttg tgtatttaaa atgatggata ggtggagatt agctgtatcg aattataaaa
      601 atcttgataa agtaacctta gatattaata aatatataca agaatctcat aaattcttaa
      661 aatttattaa aatatataca caaatagaaa atatagcaaa tgatatcgtt ttttgtcata
      721 atgatttaca agaaaataat ataatgaata ccaataaatg tttaagatta atcgattttg
      781 aatattcagg atataatttc ctatcggcag atatagcaaa cttctttata gaaacaacca
      841 tcgattattc atataacgca tatcc
//
