LOCUS       sequence-313691-       11845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2P-activatable bPAC for lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Kinjo T, Watabe T, Kobachi K, Terai K, Matsuda M
  TITLE     Single-Cell Activation of the cAMP-Signaling Pathway in 3D Tissues 
            with FRET-Assisted Two-Photon Activation of bPAC.
  JOURNAL   ACS Chem Biol. 2020 Oct 19. doi: 10.1021/acschembio.0c00333.
  PUBMED    33074647
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2020 Oct 19. doi: 10.1021/acschembio.0c00333."
COMMENT     Sequence Label: pCSIIbsr_2pabPAC
FEATURES             Location/Qualifiers
     source          1..11845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1163
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1154
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1111..1131
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(2927..3501,3502..3504,3505..3513)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3094..3111)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3321..3340
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3514..3912
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3956..4544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4009..4029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4427..4438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5063..5243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5269..5286)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5275..5499
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5545..5973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5632..5651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5842..5863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5982..6002)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5987..6316
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6167..6302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6229..6248
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6364..6411
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6430..6804
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6934..7055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6971..6990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7025..7044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7104..7120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7104..7120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7117..7139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7128..7144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7152..7158,7159..7176,7177..7182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7197..7218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7335..7352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7506..8094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7586..7605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8265..9056,9057..9125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8888..8907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9126..9230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9305..9324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9496..9875
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9877..10075
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10030..10050
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10093..10273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10320..10445
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10937..11170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11355..11399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11673..11790
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg ggcgggcctt
      361 gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
      421 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
      481 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
      541 atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
      661 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
      721 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
      781 gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg cgctcgggag
      841 agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca gccgtcgctt
      901 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tggagctttt
      961 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     1021 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     1081 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     1141 ttcttccatt tcaggtgtcg tgaacacgct accggtctcg acgaattcac catggtgagc
     1201 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
     1261 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
     1321 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
     1381 gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac
     1441 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
     1501 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
     1561 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
     1621 aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
     1681 ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag
     1741 accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc
     1801 atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg
     1861 gcccgcatga tgaagcggct ggtgtacatc agcaagatca gcggccacct gagcctggaa
     1921 gagatccagc ggatcggcaa ggtgtccatc aagaacaacc agcgggacaa catcaccggc
     1981 gtgctgctgt acctgcaggg cctgttcttc cagatcctgg aaggcgagaa cgagaaggtg
     2041 gacaagctgt acaagaaaat cctggtggac gaccggcaca ccaacatcct gtgcctgaaa
     2101 accgagtacg acatcaccga ccggatgttc cccaactggg ccatgaaaac catcaacctg
     2161 aacgagaaca gcgagctgat gatccagccc atcaagagcc tgctgcagac catcacccag
     2221 agccaccggg tgctggaaaa gtacatgccc gccagagtga tctacctgat caaccagggc
     2281 atcaaccccc tgaccgtgga gccccagctg gtcgagaaga tcatcttctt cagcgacatc
     2341 ctggccttca gcaccctgac cgagaagctg cccgtgaacg aggtggtcat cctggtcaac
     2401 cggtacttca gcatctgcac ccggatcatc agcgcctacg gcggcgaagt gaccaagttc
     2461 atcggcgact gcgtgatggc cagcttcacc aaagagcagg gcgacgccgc catccggacc
     2521 agcctggaca tcatctccga gctgaagcag ctgcggcacc acgtggaggc caccaacccc
     2581 ctgcacctgc tgtacaccgg catcggcctg agctacggcc acgtgatcga gggcaacatg
     2641 ggcagcagcc tgaagatgga ccacaccctg ctgggggacg ccgtgaacgt ggccgccagg
     2701 ctggaagccc tgacaagaca gctgccctac gccctggcct ttacagccgg cgtgaagaag
     2761 tgctgccagg cccagtggac cttcatcaac ctgggagccc accaggtcaa gggcaagcag
     2821 gaagccatcg aggtctacac cgtgaatgag gcccagaagt actacgacac cctgcagatc
     2881 acccagctga tccggcagac cctggaaaac gacaagtaag gatccgcccc tctccctccc
     2941 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3001 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3061 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3121 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     3181 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     3241 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     3301 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     3361 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat
     3421 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     3481 tttgaaaaac acgatgataa tatggccaca accatggcca agcctttgtc tcaagaagaa
     3541 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     3601 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     3661 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     3721 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     3781 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     3841 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     3901 tgggagggct aagtcgagcc tctagagtta acatcgaggg atcaagctta tcgataatca
     3961 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4021 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4081 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4141 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4201 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4261 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4321 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4381 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4441 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4501 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgaga
     4561 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     4621 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     4681 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4741 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     4801 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     4861 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     4921 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     4981 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     5041 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5221 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5281 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5341 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5401 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5461 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5521 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5581 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5641 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5701 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5761 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5821 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5881 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5941 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6001 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6061 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6121 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6241 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6301 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6361 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6421 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6481 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6541 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6601 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6661 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6721 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6781 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6841 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6901 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7081 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9241 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9301 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9361 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     9421 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     9481 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     9541 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     9601 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     9661 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     9721 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     9781 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     9841 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     9901 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     9961 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
    10021 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
    10081 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10141 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10201 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10261 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10321 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10381 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10441 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10501 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10561 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10621 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10681 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10741 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agggccgctg atcttcagac
    10801 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10861 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10921 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10981 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    11041 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    11101 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    11161 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    11221 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    11281 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    11341 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    11401 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    11461 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    11521 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    11581 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    11641 acggatctac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    11701 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    11761 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagaaattca
    11821 ctttgaatta attcaagctt cgtga
//
